Effects of synthetic kisspeptin peptides and GnRH analogue on oocyte growth and circulating sex steroids in prepubertal female chub mackerel (Scomber japonicus)
Effects of synthetic kisspeptin peptides and GnRH analogue on oocyte growth and circulating sex steroids in prepubertal female chub mackerel (Scomber japonicus)
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DOI：
10.1111/are.12342
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发表时间：
2015-08
影响因子：
2
通讯作者：
S. Selvaraj;H. Ohga;Mitsuo Nyuji;Hajime Kitano;N. Nagano;A. Yamaguchi;M. Matsuyama
中科院分区：
文献类型：
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作者：
S. Selvaraj;H. Ohga;Mitsuo Nyuji;Hajime Kitano;N. Nagano;A. Yamaguchi;M. Matsuyama
近年来，由kiss基因编码的kisspeptin肽已被用于操纵养殖动物（包括鱼类）的生殖过程。我们以前的研究表明，鲭鱼脑表达kiss 1和kiss 2，肌肉注射合成的Kiss 1十五肽（Kiss 1 -15），而不是Kiss 2十二肽（Kiss 2 -12）促进精子发生在青春期前的雄性鲭鱼（Scomber）。在本研究中，我们评估了他们的影响，在青春期前的女性鲢鱼。实验鱼的性腺指数（GSI）值没有表现出任何显着的变化。条件因子（CF）值显着增加Kiss 1 -15处理的鱼，与对照组和GnRH类似物（GnRHa）注射鱼。在组织学上，所有实验鱼中只发现了核仁周卵母细胞。然而，吻和GnRHa治疗的鱼表现出显着增加的核仁周围的卵母细胞直径，与对照鱼相比。基因表达分析显示，与对照鱼相比，注射Kiss 2 -12和GnRHa的鱼的端脑视前区中GnRH 1的表达降低。相比之下，注射GnRHa的鱼在垂体中表现出更高的fshβ水平，而lhβ水平在不同处理之间没有变化。与对照鱼相比，注射Kiss 1 -15的鱼的循环性类固醇、睾酮和雌二醇-17 β的水平显著更高。这些结果表明，合成的kisspeptin肽和GnRHa可以诱导卵母细胞的生长在青春期前的雌性鲭鱼。
In recent years, kisspeptin peptides, encoded by kiss genes have been used to manipulate reproductive processes in farmed animals, including fish. Our previous studies demonstrated that the chub mackerel brain expresses kiss1 and kiss2 and intramuscular injection of synthetic Kiss1 pentadecapeptide (Kiss1-15) but not Kiss2 dodecapeptide (Kiss2-12) accelerates spermatogenesis in prepubertal male chub mackerel (Scomber japonicus). In the present study, we evaluated their effects in prepubertal female chub mackerel. The gonadosomatic index (GSI) values of experimental fish did not show any significant changes. Condition factor (CF) values increased significantly in Kiss1-15 treated fish, in comparison with control and GnRH analogue (GnRHa) injected fish. Histologically, only perinucleolar oocytes were found in all experimental fish. However, Kiss and GnRHa treated fish showed a significant increase in the perinucleolar oocyte diameter, in comparison with the control fish. Gene expression analyses revealed decreased expression of gnrh1 in the telencephalon-preoptic region of the brain of Kiss2-12 and GnRHa injected fish, in comparison with control fish. In contrast, GnRHa injected fish exhibited higher levels of fshβ in the pituitary, with no changes in the levels of lhβ among different treatments. Levels of circulating sex steroids, testosterone, and estradiol-17β were significantly higher in Kiss1-15 injected fish, in comparison with control fish. These results indicate that synthetic kisspeptin peptides and GnRHa can induce oocyte growth in prepubertal female chub mackerel.