OLFACTORY RELATIONSHIPS OF TELENCEPHALON AND DIENCEPHALON IN RABBIT .3. IPSILATERAL CENTRIFUGAL FIBERS TO OLFACTORY BULBAR AND RETROBULBAR FORMATIONS
OLFACTORY RELATIONSHIPS OF TELENCEPHALON AND DIENCEPHALON IN RABBIT .3. IPSILATERAL CENTRIFUGAL FIBERS TO OLFACTORY BULBAR AND RETROBULBAR FORMATIONS
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DOI：
10.1002/cne.901700305
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发表时间：
1976-01-01
影响因子：
2.5
通讯作者：
JACOBOWITZ, DM
中科院分区：
文献类型：
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作者：
BROADWELL, RD;JACOBOWITZ, DM
用辣根过氧化物酶(HRP)从轴突终末向其亲本细胞体的轴浆逆行运输和组织化学荧光显微镜技术，研究了兔同侧向嗅球和嗅前核的离心性纤维。将过氧化物酶单侧注入主、副嗅球或嗅前核内。在主球内注射HRP的动物，在同侧前脑的梨状前皮质、斜角带核的水平支、下丘脑远外侧视前区和吻侧外侧区可见逆行标记的核膜。含有HRP阳性胞体的脑干细胞群包括蓝斑和中脑中缝背核。除筛前皮质外，基底前脑结构的核膜标记与含有乙酰胆碱酯酶和胆碱乙酰转移酶的细胞体的位置密切相关。这些胞体可能代表主要嗅球的胆碱能传入系统。蓝斑和中脑中缝的过氧化物酶标记胞体分别代表嗅球的去甲肾上腺素能和5-羟色胺能神经支配。在将过氧化物酶注射或扩散到副嗅球和前嗅核的兔中，在双侧梨状前皮质、斜角带核的水平支、下丘脑外侧区、外侧嗅束核、杏仁皮质内侧复合体、同侧主嗅球的二尖瓣细胞层和簇状细胞层、蓝斑和中脑中缝均可见HRP阳性胞体。文中还讨论了杏仁核皮质内侧复合体、蓝斑以及可能的外侧嗅束核和中缝核投射到副嗅球的证据。在将过氧化物酶注射到主嗅球并扩散到副球和前嗅核的大鼠中，前脑和脑干中标记的核膜也有类似的分布。兔和大鼠主、副嗅球的组织化学荧光显微镜下可见细小口径、绿色荧光纤维和突起，以颗粒细胞层为主，肾小球层细胞较少。在主嗅球根部切片中，在前嗅核外部丛状层的深部可见类似的荧光。这些荧光纤维可能代表嗅球和球后结构的去甲肾上腺素能神经支配。在主球的肾小球层中观察到荧光黄色，这可能表示该板层的5-羟色胺能神经支配。这证实了主嗅觉系统和副嗅觉系统的存在，并为嗅觉大脑的组织以及下丘脑和嗅觉的相互关系提供了更多的洞察力。
The axoplasmic retrograde transport of horseradish peroxidase (HRP) from axon terminals to their parent cell bodies and histochemical fluorescence microscopy were used to study the ipsilateral centrifugal fibers to the olfactory bulbs and anterior olfactory nucleus in the rabbit. Focal injections of peroxidase were placed unilaterally into the main or accessory olfactory bulb or into the anterior olfactory nucleus. In animals with injected HRP confined within the main bulb, perikarya retrogradely labeled with the protein in the ipsilateral forebrain were observed in the anterior prepyriform cortex, horizontal limb of the nucleus of the diagonal band, and far lateral preoptic and rostral lateral hypothalamic areas. Brain stem cell groups that contained HRP-positive somata include the locus coeruleus and midbrain dorsal raphe nucleus. Except for the prepyriform cortex, the basal forebrain structures with labeled perikarya correlate well with locations of cell bodies containing acetylcholinesterase and choline acetyltransferase. These somata may represent a cholinergic afferent system to the main olfactory bulb. Peroxidase-labeled cell bodies in the locus coeruleus and midbrain raphe are indicative of noradrenergic and serotonergic innervations respectively of the olfactory bulb. In rabbits in which peroxidase was injected or diffused into the accessory olfactory bulb and anterior olfactory nucleus, HRP-positive somata were identified in the prepyriform cortex bilaterally, the horizonal limb of the diagonal band nucleus, lateral hypothalamic region, nucleus of the lateral olfactory tract, corticomedial complex of the amygdala, mitral and tufted cell layers of the ipsilateral main olfactory bulb, locus coeruleus and the midbrain raphe. Evidence for centrifugal fibers to the accessory olfactory bulb from the corticomedial complex of the amygdala, locus coeruleus and possibly the nucleus of the lateral olfactory tract and midbrain raphe was discussed. A similar distribution of labeled perikarya in the forebrain and brain stem was seen in rats in which peroxidase injected into the main olfactory bulb had spread into the accessory bulb and anterior olfactory nucleus. Histochemical fluorescence microscopy of the main and accessory olfactory bulbs in the rabbit and rat revealed fine caliber, green fluorescent fibers and varicosities predominantly in the granule cell layer and less so among cells in the glomerular layer. In sections through the root of the main olfactory bulb, a similar fluorescence was seen in the deep half of the plexiform layer of the pars externa of the anterior olfactory nucleus. These fluorescent fibers likely represent the noradrenergic innervation of the olfactory bulbar and retrobulbar formations. A fluorescent yellow hue was observed in the glomerular layer of the main bulb and may signify a serotonergic innervation of this lamina. This confirms the existence of main and accessory olfactory systems and offers greater insight into the organization of the olfactory brain and the interrelationship of the hypothalamus and olfaction.