Asthma and Chronic Bronchitis
Asthma and Chronic Bronchitis
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哮喘和慢性支气管炎
DOI：
10.1136/bmj.2.3837.139-a
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发表时间：
1934
影响因子：
--
通讯作者：
J. B. Christopherson
中科院分区：
文献类型：
--
作者：
J. B. Christopherson
检查了痰细胞计数的重复性，并将哮喘患者的细胞计数与慢性支气管炎患者的细胞计数进行了比较。研究了三组受试者。研究了八名有痰液的慢性哮喘患者的痰液，以确定痰细胞计数的重现性。将 10 名在咳痰恶化时不并发其他气道疾病的哮喘非吸烟者（第 2 组）的结果与 8 名患有慢性咳嗽和咳痰但无哮喘特征的吸烟者（第 3 组）的结果进行比较。通过显微镜选择痰塞以确保其源自下呼吸道。对胰蛋白酶处理的悬浮液进行总细胞计数，对未稀释的细胞块进行差异和异染细胞计数。这些测量的样本内和重测重现性很高（可靠性系数 R = 099 和 0O89）。哮喘患者的痰液特征为嗜酸性粒细胞增多（69%，范围46-92%）和存在甲醛可阻断的异染细胞（1-5%，范围0'6-2-8%）。相比之下，慢性支气管炎患者的痰中嗜酸性粒细胞（0-5%）或异染细胞（0'14%）很少；主要细胞类型是巨噬细胞（83%）。结论：痰细胞计数在短期内具有可重复性，哮喘炎症以痰液中嗜酸性粒细胞增多和异染细胞为特征，痰液可能为研究气道炎症的细胞特征提供有用的细胞来源。
The reproducibility of sputum cell counts was examined and the cell counts in patients with asthma were compared with those in patients with chronic bronchitis. Three groups of subjects were studied. Sputum from eight patients with chronic asthma and with sputum production were studied to determine the reproducibility of sputum cell counts. The findings in 10 non-smokers with asthma uncomplicated by other airway disease examined at the time ofan exacerbation with sputum (group 2) were compared with those from eight smokers with chronic cough and sputum but no features of asthma (group 3). Sputum plugs were selected by microscopy to ensure their origin from the lower respiratory tract. A total cell count was performed on a trypsinised suspension, and differential and metachromatic cell counts were performed on undiluted plugs. The within specimen and test-retest reproducibility of these measurements was high (reliability coefficient, R, = 099 and 0O89). The sputum of the asthmatic patients was characterised by eosinophilia (69%, range 46-92%) and the presence of formaldehyde blockable metachromatic cells (1-5%, range 0'6-2-8%). In comparison, the sputum of the patients with chronic bronchitis had few eosinophils (0-5%) or metachromatic cells (0' 14%); the dominant cell type was the macrophage (83%). It is concluded that sputum cell counts are reproducible in the short term, the inflammation ofasthma is characterised by eosinophilia and metachromatic cells in sputum, and sputum may provide a useful source of cells for investigating the cellular characteristics of airway inflammation.