Efficient Homogenization-Ultrasound-Assisted Extraction of Anthocyanins and Flavonols from Bog Bilberry (Vaccinium uliginosum L.) Marc with Carnosic Acid as an Antioxidant Additive
Efficient Homogenization-Ultrasound-Assisted Extraction of Anthocyanins and Flavonols from Bog Bilberry (Vaccinium uliginosum L.) Marc with Carnosic Acid as an Antioxidant Additive
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以鼠尾草酸作为抗氧化添加剂，高效均质超声辅助从沼泽越橘 (Vaccinium uliginosum L.) 果渣中提取花青素和黄酮醇
DOI：
10.3390/molecules24142537
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发表时间：
2019-07-02
期刊：
影响因子：
4.6
通讯作者：
Zhou, Zhiqiang
中科院分区：
文献类型：
--
作者：
Jin, Yusong;Zhang, Yunhui;Zhou, Zhiqiang
采用响应面法，在单因素试验的基础上，探索山越橘中花青素和黄酮醇的最佳提取条件。提取过程在沃林搅拌器中进行，超声波浴后，加入天然抗氧化剂鼠尾草酸抑制氧化。最佳提取条件为：乙醇体积分数为70%，抗氧化剂含量为0.02%（样品质量）鼠尾草酸，液固比为16 mL/g，均质时间3 min，反应温度55℃，超声照射频率80 kHz，超声照射功率200 W，超声照射时间40 min。花青素（13.95 +/- 0.37 mg/g）和黄酮醇（3.51 +/- 0.16 mg/g）的产率令人满意。实验结果表明，鼠尾草酸在花青素和黄酮醇的提取过程中具有有效的抗氧化作用，具有绿色、安全的保证。
To explore the optimum conditions for the extraction of anthocyanins and flavonols from bog bilberry (Vaccinium uliginosum L.) marc on a single-factor experimental basis, a response surface methodology was adopted for this intensive study. The extraction procedure was carried out in a Waring blender and followed an ultrasonic bath, and the natural antioxidant carnosic acid was added to inhibit oxidation. The optimum extraction conditions were as follows: a volume fraction of ethanol of 70%, an antioxidant content of 0.02% (the mass of sample) carnosic acid, a liquid-solid ratio of 16 mL/g, a homogenization time of 3 min, a reaction temperature of 55 degrees C, an ultrasound irradiation frequency of 80 kHz, an ultrasound irradiation power of 200 W, and an ultrasound irradiation time of 40 min. Satisfactory yields of anthocyanins (13.95 +/- 0.37 mg/g) and flavonols (3.51 +/- 0.16 mg/g) were obtained. The experimental results showed that the carnosic acid played an effective antioxidant role in the extraction process of anthocyanins and flavonols with a green and safety guarantee.