Epidermal stem cells arise from the hair follicle after wounding
Epidermal stem cells arise from the hair follicle after wounding
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DOI：
10.1096/fj.06-6926com
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发表时间：
2007-05-01
期刊：
影响因子：
4.8
通讯作者：
Morgan, Bruce A.
中科院分区：
文献类型：
--
作者：
Levy, Vered;Lindon, Catherine;Morgan, Bruce A.
在正常发育过程中，表皮和毛囊是由独立的干细胞群维持的不同谱系区域。表皮和毛囊的角质形成细胞都会在受伤时被招募参与表皮修复。然而，人们普遍认为毛囊细胞只是短暂地参与伤口表皮的形成，最终会被受伤前来自原始表皮区域的干细胞后代所取代。在此，我们利用从音猬因子基因座（Shh）表达的诱导型和组成型cre重组酶进行体内谱系追踪。这一分析证实毛囊细胞参与伤口的初始重新上皮化，但也揭示它们的后代在伤口愈合后数月仍存在于伤口表皮中。它进一步表明在伤口愈合过程中角质形成细胞中的Shh未被诱导。我们得出结论，毛囊细胞在受伤后可经历重编程，成为长期重新增殖的表皮祖细胞。
During normal development, the epidermis and hair follicle are distinct lineage compartments maintained by independent stem cell populations. Both epidermal and follicular keratinocytes are recruited to participate in epidermal repair in response to injury. However, it is generally thought that follicular cells contribute to the wound epidermis only transiently and are ultimately replaced by the progeny of stem cells derived from the original epidermal compartment prior to wounding. Here we use inducible and constitutive cre recombinase expressed from the Sonic hedgehog locus ( Shh) for in vivo lineage tracing. This analysis confirms that follicular cells participate in the initial resurfacing of the wound but also reveals that their progeny persist in wound epidermis for months after the wound is healed. It further demonstrates that Shh is not induced in keratinocytes during the wound healing process. We conclude that follicular cells can undergo reprogramming to become long-term repopulating epidermal progenitors following wounding.