Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis
Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis
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DOI：
10.1099/vir.0.2008/001503-0
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发表时间：
2008-09-01
影响因子：
3.8
通讯作者：
Buonavoglia, C.
中科院分区：
文献类型：
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作者：
Decaro, N.;Desario, C.;Buonavoglia, C.
从意大利（n=34）和英国（n=5）收集的猫泛白细胞减少症猫粪便样本中含有39株细小病毒株，在分子水平上进行了表征。通过小沟结合物探针试验证明所有病毒都是真正的猫泛白细胞减少症病毒（FPLV）毒株，该试验能够区分FPLV和密切相关的犬细小病毒2型。通过对VP 2基因序列的分析，发现意大利和英国的FPLV毒株之间有很高的同源性，意大利株和英国株之间的核苷酸同源性分别为99.1- 100%和99.4-99.8%，而所有序列之间的相似性为98.6- 100%。在VP 2基因上共检测到88个变异位点，其中大部分为单基因变异。同义替换（n=57）比非同义替换（n=31）占优势，同义替换与非同义替换之比（dN/dS）为0.10，证实FPLV的进化是由随机遗传漂变而不是正选择压力驱动的。VP 2蛋白中的一些氨基酸突变影响了被认为负责病毒抗原和生物学特性的位点，但没有明确的分离模式和遗传标记，证实了FPLV处于进化停滞状态。
Thirty-nine parvovirus strains contained in faecal samples collected in Italy (n=34) and UK (n=5) from cats with feline panleukopenia were characterized at the molecular level. All viruses were proven to be true feline panleukopenia virus (FPLV) strains by a minor groove binder probe assay, which is able to discriminate between FPLV and the closely related canine parvovirus type 2. By using sequence analysis of the VP2 gene, it was found that the FPLV strains detected in Italy and UK were highly related to each other, with a nucleotide identity of 99.1-100 and 99.4-99.8% among Italian and British strains, respectively, whereas the similarities between all the sequences analysed were 98.6-100%. Eighty-eight variable positions were detected in the VP2 gene of the field and reference FPLV strains, most of which were singletons. Synonymous substitutions (n=57) predominated over non-synonymous substitutions (n=31), and the ratio between synonymous and non-synonymous substitutions (dN/dS) was 0.10, thus confirming that evolution of FPLV is driven by random genetic drift rather than by positive selection pressure. Some amino acid mutations in the VP2 protein affected sites that are thought to be responsible for antigenic and biological properties of the virus, but no clear patterns of segregation and genetic markers, were identified, confirming that FPLV is in evolutionary stasis.