A bionic system with Fenton reaction and bacteria as a model for bioprocessing lignocellulosic biomass.
A bionic system with Fenton reaction and bacteria as a model for bioprocessing lignocellulosic biomass.
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以芬顿反应和细菌为模型的仿生系统用于木质纤维素生物质的生物加工
DOI：
10.1186/s13068-018-1035-x
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发表时间：
2018
影响因子：
6.3
通讯作者：
Shi Y
中科院分区：
文献类型：
--
作者：
Zhang K;Si M;Liu D;Zhuo S;Liu M;Liu H;Yan X;Shi Y
木质纤维素生物质的可再生性为生物燃料和生物制品的生物化学加工提供了一系列挑战。我们首次通过温和而快速的芬顿反应和木质纤维素降解菌株Cupriavidus basilensis B-8（以下简称B-8）来模拟自然真菌入侵过程，用仿生系统预处理稻草（RS）。结果芬顿和B-8协同预处理后，还原糖产量比单独芬顿预处理提高15.6-56.6%，比未处理RS（98 mg g-1）提高2.7-5.2倍。形态学分析表明，预处理改变了RS的表面形态，粗糙度和亲水位点的增加增强了木质纤维素的生物利用度。化学成分分析表明，B-8去除了部分木质素和半纤维素，使纤维素含量增加。此外，木质素也发生了重要的化学改性，2D NMR分析表明，芬顿预处理主要通过切断β-O-4键和脱甲氧基作用去除愈创木基（S）和愈创木基（G）单元，使木质素发生部分解聚。B-8可通过切断连接木质素亚基的β-5键来减少G单元的数量.ConclusionsA仿生系统与生化芬顿反应和木质纤维素降解菌被证实能够用于RS的预处理，以在温和的条件下增强酶解。高消化率归因于木质素结构的破坏、半纤维素的部分水解和纤维素的部分表面氧化。探讨了芬顿和B-8协同预处理的机理，以了解RS的变化和细菌对酶解的影响。此外，这种仿生系统为木质纤维素生物质的预处理提供了新的见解。
BackgroundThe recalcitrance of lignocellulosic biomass offers a series of challenges for biochemical processing into biofuels and bio-products. For the first time, we address these challenges with a biomimetic system via a mild yet rapid Fenton reaction and lignocellulose-degrading bacterial strainCupriavidus basilensisB-8 (here after B-8) to pretreat the rice straw (RS) by mimicking the natural fungal invasion process. Here, we also elaborated the mechanism through conducting a systematic study of physicochemical changes before and after pretreatment.ResultsAfter synergistic Fenton and B-8 pretreatment, the reducing sugar yield was increased by 15.6–56.6% over Fenton pretreatment alone and 2.7–5.2 times over untreated RS (98 mg g−1). Morphological analysis revealed that pretreatment changed the surface morphology of the RS, and the increase in roughness and hydrophilic sites enhanced lignocellulose bioavailability. Chemical components analyses showed that B-8 removed part of the lignin and hemicellulose which caused the cellulose content to increase. In addition, the important chemical modifications also occurred in lignin, 2D NMR analysis of the lignin in residues indicated that the Fenton pretreatment caused partial depolymerization of lignin mainly by cleaving the β-O-4 linkages and by demethoxylation to remove the syringyl (S) and guaiacyl (G) units. B-8 could depolymerize amount of the G units by cleaving the β-5 linkages that interconnect the lignin subunits.ConclusionsA biomimetic system with a biochemical Fenton reaction and lignocellulose-degrading bacteria was confirmed to be able for the pretreatment of RS to enhance enzymatic hydrolysis under mild conditions. The high digestibility was attributed to the destruction of the lignin structure, partial hydrolysis of the hemicellulose and partial surface oxidation of the cellulose. The mechanism of synergistic Fenton and B-8 pretreatment was also explored to understand the change in the RS and the bacterial effects on enzymatic hydrolysis. Furthermore, this biomimetic system offers new insights into the pretreatment of lignocellulosic biomass.
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影响因子：
6.3
作者：
Banerjee G;Car S;Scott-Craig JS;Hodge DB;Walton JD
通讯作者：
Walton JD
影响因子：
3.8
作者：
Ko, Ja Kyong;Ximenes, Eduardo;Ladisch, Michael R.
通讯作者：
Ladisch, Michael R.
影响因子：
3.4
作者：
Bhange VP;William SP;Sharma A;Gabhane J;Vaidya AN;Wate SR
通讯作者：
Wate SR
影响因子：
10.4
作者：
Saha, Shouvik;Kurade, Mayur B.;Jeon, Byong-Hun
通讯作者：
Jeon, Byong-Hun
影响因子：
9.8
作者：
Salvachua, Davinia;Katahira, Rui;Beckham, Gregg T.
通讯作者：
Beckham, Gregg T.