Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features.
Dyssynchronous heart failure models in canines: New insights into electrocardiographic, echocardiographic and histological features.
复制标题
犬的不同步心力衰竭模型：对心电图、超声心动图和组织学特征的新见解。
DOI：
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发表时间：
2024
影响因子：
3.7
通讯作者：
Hui Li
中科院分区：
文献类型：
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作者：
Han Jin;Shengwen Yang;Hao Huang;S. Cheng;Pengkang He;Sixian Weng;M. Gu;H. Niu;Wei Hua;Yiran Hu;Hui Li
背景
我们研究了两种实验方法之间的异同，使用快速起搏技术，以诱导犬去心肌化心力衰竭。
方法
8只犬，消融左束支分支，建立左束支分支阻滞（RAP+LBBB）模型，其中4只在右心室心尖部快速起搏（RVAP），4只在右心房快速起搏。随访三周，观察心功能变化，实验结束时进行心肌染色。
结果
两种实验方法都成功地建立了射血分数降低的心力衰竭模型，心脏功能下降的趋势相似。RAP+LBBB组表现出总体心室激动时间延长，左心室激动延迟，对右心室的影响较小。RVAP方法导致整体右心室顺应性降低和右心室扩大。RAP+LBBB组的左心室顺应性显著降低，（LVGLS，%：RAP+LBBB-12.60 ± 0.12至-5.93 ± 1.25; RVAP-13.28 ± 0.62至-8.05 ± 0.63，p = 0.023; LASct，%：RAP+LBBB-15.75 ± 6.85至-1.50 ± 1.00; RVAP -15.75 ± 2.87至-10.05 ± 6.16，p = 0.035）。组织学检查显示RAP+LBBB组的左心室壁和左心房纤维化更明显，而RVAP组的右心室心肌纤维化更明显。
结论
这两种方法都建立了具有可比趋势的HFrEF模型。RVAP组右心室功能受损，而RAP+LBBB组左心室顺应性降低和纤维化程度更严重。
BACKGROUND
We investigated the similarities and differences between two experimental approaches using tachy-pacing technology to induce desynchronized heart failure in canines.
METHODS
A total of eight dogs were included in the experiment, four were tachy-paced in right ventricle apex (RVAP) and 4 were paced in right atrium after the ablation of left bundle branch to achieve left bundle branch block (RAP+LBBB). Three weeks of follow-up were conducted to observe the changes in cardiac function and myocardial staining was performed at the end of the experiment.
RESULTS
Both experimental approaches successfully established heart failure with reduced ejection fraction models, with similar trends in declining cardiac function. The RAP+LBBB group exhibited a prolonged overall ventricular activation time, delayed left ventricular activation, and lesser impact on the right ventricle. The RVAP approach led to a reduction in overall right ventricular compliance and right ventricular enlargement. The RAP+LBBB group exhibited significant reductions in left heart compliance (LVGLS, %: RAP+LBBB -12.60 ± 0.12 to -5.93 ± 1.25; RVAP -13.28 ± 0.62 to -8.05 ± 0.63, p = 0.023; LASct, %: RAP+LBBB -15.75 ± 6.85 to -1.50 ± 1.00; RVAP -15.75 ± 2.87 to -10.05 ± 6.16, p = 0.035). Histological examination revealed more pronounced fibrosis in the left ventricular wall and left atrium in the RAP+LBBB group while the RVAP group showed more prominent fibrosis in the right ventricular myocardium.
CONCLUSION
Both approaches establish HFrEF models with comparable trends. The RVAP group shows impaired right ventricular function, while the RAP+LBBB group exhibits more severe decreased compliance and fibrosis in left ventricle.