The Effects of Irisin on Nω-Nitro-L-arginine Methyl Ester Hydrochloride-Induced Hypertension in Rats
The Effects of Irisin on Nω-Nitro-L-arginine Methyl Ester Hydrochloride-Induced Hypertension in Rats
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DOI：
10.4274/balkanmedj.galenos.2019.2019.5.113
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发表时间：
2019-11-01
影响因子：
3
通讯作者：
Kandemir, Nihayet
中科院分区：
文献类型：
--
作者：
Aydogdu, Nurettin;Yavuz, Ozlem Yalcinkaya;Kandemir, Nihayet
背景：高血压是一个重要的公共卫生问题，约95%的高血压病因不明。深入的研究正在进行中，以了解高血压的生理病理。鸢尾素是一种新发现的激素，据报道，它能迅速扩张血管平滑肌，降低血压。目的：观察慢性鸢尾素治疗对硝酸合酶抑制型高血压模型血压和肾功能的影响。研究设计：动物实验。方法：雄性Sprague-Dawley大鼠分为4组（n=8）。对照组和鸢尾素组静脉注射生理盐水，高血压组和高血压+鸢尾素（高血压+鸢尾素）组静脉注射1.5 mg/100 g N ω -硝基- l -精氨酸甲酯盐酸盐。在高血压组和高血压+鸢尾素组大鼠的饮用水中添加N - omega-硝基-L-精氨酸甲酯盐酸盐（150 mg/L），持续3周。实验第2周，鸢尾素组和高血压+鸢尾素组大鼠给予鸢尾素（50 nmol/d），对照组和高血压组大鼠通过皮下放置渗透微型泵给予生理盐水，持续2周。采用尾袖容积描记法测量血压。实验第21天采集大鼠24小时尿液、血液及双肾。结果：与对照组相比，高血压组的收缩压、舒张压和平均动脉血压升高，组织和血清中谷胱甘肽水平降低，但血清中氧化谷胱甘肽水平升高(p
Background: The cause of about 95% of hypertension, an important public health problem, is unknown. Intensive studies are underway to understand the physiopathology of hypertension. Irisin, a newly discovered hormone, has been reported to dilate vascular smooth muscle and lower blood pressure acutely.Aims: To investigate the effects of chronic irisin treatment on blood pressure and renal functions in a hypertension model established by nitric oxide synthase inhibition by treatment with N omega-nitro-L-arginine methyl ester hydrochloride.Study Design: Animal experimentation.Methods: Male Sprague-Dawley rats were divided into four groups (n=8). Control and irisin groups received an intravenous saline injection, hypertension and hypertension + irisin (hypertension + irisin) groups received 1.5 mg/100 g N omega-nitro-L-arginine methyl ester hydrochloride. N omega-nitro-L-arginine methyl ester hydrochloride (150 mg/L) was added to the drinking water of rats in groups hypertension and hypertension + irisin for three weeks. In the second week of the experiment, irisin (50 nmol/day) was given to rats in groups irisin and hypertension + irisin, and saline was administered to rats in groups control and hypertension for two weeks through subcutaneously placed osmotic minipumps. Blood pressure was measured by the tail-cuff plethysmography method. On the twenty-first day of the experiment, 24-hour urine, blood, and both kidneys of the rats were collected.Results: The hypertension group had elevated systolic, diastolic, and mean arterial blood pressure values compared with the control group, with decreased glutathione levels in tissue and serum, but an increase in serum oxidized glutathione level (p