Regional influences on the physical properties of T cell membranes.
Regional influences on the physical properties of T cell membranes.
复制标题
T 细胞膜物理特性的区域影响。
DOI：
10.1016/0024-3205(91)90499-2
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发表时间：
1991
期刊：
影响因子：
6.1
通讯作者：
Santoro,TJ
中科院分区：
文献类型：
--
作者：
Tomita-Yamaguchi,M;Rubio,C;Santoro,TJ
来自不同组织的细胞之间膜脂组成的差异已得到充分记录。这些差异可能通过质膜内脂质分子的运动自由度或流动性的变化来体现，并且可能导致细胞功能的改变。来自小鼠脾脏和淋巴结的胸腺衍生细胞对抗原引发的不同反应的发现暗示了对免疫功能的区域影响。微环境也塑造 T 淋巴细胞膜的物理特性的可能性尚未被探索，这是本研究的重点。使用小鼠作为实验模型，发现免疫正常小鼠和易患自身免疫的MRL-1pr/1pr小鼠的脾和淋巴结T细胞之间的流动性和细胞内游离离子化Ca 2+ 的静息水平存在差异。结果表明，T 细胞比之前认识的更加异质，并表明微环境在决定免疫反应中的潜在作用。
Differences in the composition of membrane lipids are well documented between cells from distinct tissues. These differences may be manifested by changes in the motional freedom or fluidity of lipid molecules within plasma membranes and may predispose to alterations in cellular function. Regional influences on immune function have been implied by the finding that thymic-derived cells from murine spleen and lymph nodes are differentially responsive to antigen priming. The possibility that microenvironment also shapes the physical properties of T lymphocyte membranes has not been explored and is the focus of this study. Using mice as the experimental model, differences were found in fluidity and in the resting level of intracellular free-ionized Ca 2+ between splenic and lymph node T cells from immunologically normal mice and from autoimmune-prone MRL-1pr/1pr mice. The results indicate that T cells are more heterogeneous than previously recognized and suggest a potential role for microenvironment in determining immune responsiveness.