Morning and evening peaks of activity rely on different clock neurons of the Drosophila brain
Morning and evening peaks of activity rely on different clock neurons of the Drosophila brain
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DOI：
10.1038/nature02935
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发表时间：
2004-10-14
期刊：
影响因子：
64.8
通讯作者：
Rouyer, F
中科院分区：
文献类型：
--
作者：
Grima, B;Chèlot, E;Rouyer, F
在果蝇中，位于大脑中的“时钟”控制着运动活动的昼夜节律。这种时钟依赖于表达周期（PER）蛋白的几组神经元，包括表达色素分散因子（PDF）神经肽的腹侧神经元（LN(v)s）和PDF阴性的背侧神经元（LN(d)s）(1)。在正常昼夜循环中，成虫的活动高峰为早晚（1,2）；然而，不同的时钟神经元对休息活动模式的贡献仍然未知。在这里，我们使用PER的靶向表达来恢复心律失常的PER 0突变果蝇中特定侧神经元亚群的时钟功能。我们发现，仅在LNvs中表达PER只能恢复早晨的活动，而在LNvs和LNds中表达PER也可以恢复晚上的活动。这为果蝇大脑中的“早晨”和“晚上”振荡提供了第一个神经元基础。此外，我们发现LNvs可以在持续的黑暗中产生24小时的活动节律，这表明早晨振荡器足以驱动昼夜节律系统。
In Drosophila, a 'clock' situated in the brain controls circadian rhythms of locomotor activity. This clock relies on several groups of neurons that express the Period ( PER) protein, including the ventral lateral neurons (LN(v)s), which express the Pigment-dispersing factor (PDF) neuropeptide, and the PDF-negative dorsal lateral neurons (LN(d)s)(1). In normal cycles of day and night, adult flies exhibit morning and evening peaks of activity(1,2); however, the contribution of the different clock neurons to the rest activity pattern remains unknown. Here, we have used targeted expression of PER to restore the clock function of specific subsets of lateral neurons in arrhythmic per 0 mutant flies. We show that PER expression restricted to the LNvs only restores the morning activity, whereas expression of PER in both the LNvs and LNds also restores the evening activity. This provides the first neuronal bases for 'morning' and 'evening' oscillators in the Drosophila brain. Furthermore, we show that the LNvs alone can generate 24 h activity rhythms in constant darkness, indicating that the morning oscillator is sufficient to drive the circadian system.