A NAPHTHYL ANALOG OF THE AMINOSTYRYL PYRIDINIUM CLASS OF POTENTIOMETRIC MEMBRANE DYES SHOWS CONSISTENT SENSITIVITY IN A VARIETY OF TISSUE, CELL, AND MODEL MEMBRANE PREPARATIONS
A NAPHTHYL ANALOG OF THE AMINOSTYRYL PYRIDINIUM CLASS OF POTENTIOMETRIC MEMBRANE DYES SHOWS CONSISTENT SENSITIVITY IN A VARIETY OF TISSUE, CELL, AND MODEL MEMBRANE PREPARATIONS
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DOI：
10.1007/bf00233734
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发表时间：
1992-10-01
影响因子：
2.4
通讯作者：
WU, JY
中科院分区：
文献类型：
--
作者：
LOEW, LM;COHEN, LB;WU, JY
快速电位指示剂di-4-ANEPPS在脂质囊泡、红细胞、鱿鱼巨轴突和豚鼠心脏四种不同的制剂中进行了检测。染料在这些系统中给出一致的电位响应，尽管一些详细的行为有所不同。在脂质囊泡中，染料显示荧光增加，并伴有超极化激发光谱的红移。在红细胞中也发现了类似的行为，其中双波长比率测量也被证明。电位荧光反应的信噪比是在电压夹住的鱿鱼轴突上记录的最好的。该染料被证明是一种忠实和持久的心脏动作电位监测仪，在每10分钟间歇照明长达2小时的时间内，没有明显的信号损失或心脏活动恶化。这些结果，加上之前发表的染料在球形脂质双层模型和培养细胞中的应用，证明了di-4-ANEPPS作为膜电位快速指标的多功能性。
The fast potentiometric indicator di-4-ANEPPS is examined in four different preparations: lipid vesicles, red blood cells, squid giant axon, and guinea pig heart. The dye gives consistent potentiometric responses in each of these systems, although some of the detailed behavior varies. In lipid vesicles, the dye displays an increase in fluorescence combined with a red shift of the excitation spectrum upon hyperpolarization. Similar behavior is found in red cells where a dual wavelength ratiometric measurement is also demonstrated. The signal-to-noise ratio of the potentiometric fluorescence response is among the best ever recorded on the voltage-clamped squid axon. The dye is shown to be a faithful and persistent monitor of cardiac action potentials with no appreciable loss of signal or deterioration of cardiac activity for periods as long as 2 hr with intermittent illumination every 10 min. These results, together with previously published applications of the dye to a spherical lipid bilayer model and to cells in culture, demonstrate the versatility of di-4-ANEPPS as a fast indicator of membrane potential.