ADJACENT LAMINAR TERMINATIONS OF 2 CENTRIFUGAL AFFERENT PATHWAYS TO THE ACCESSORY OLFACTORY-BULB IN THE MOUSE
ADJACENT LAMINAR TERMINATIONS OF 2 CENTRIFUGAL AFFERENT PATHWAYS TO THE ACCESSORY OLFACTORY-BULB IN THE MOUSE
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DOI：
10.1016/0006-8993(82)90803-4
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发表时间：
1982-01-01
期刊：
影响因子：
2.9
通讯作者：
BARBER, PC
中科院分区：
文献类型：
--
作者：
BARBER, PC
采用顺行和逆行轴突追踪结合终纹横断的方法，研究了小鼠副嗅球的离心性传入纤维联系。将~ 3 H-脯氨酸注射到杏仁皮质后内侧核（C3），引起顺行放射自显影标记的通路终止于副嗅球（AOB）的内部颗粒层。横断同侧的终纹完全废除了这一途径的标记。进一步吻侧注射，在副嗅束（BNAOT）和内侧杏仁核（M）的床核，引起标记的第二同侧传入通路的AOB终止于内部丛状层（IPL），不受大脑皮层横断。将麦胚凝集素-HRP [辣根过氧化物酶]结合物注射到AOB中证实，它接收来自同侧BR AOT、M和C3以及来自对侧C3中的一些细胞的传入。横断同侧纹状体终末可防止同侧C3中任何细胞的逆行标记，但不影响M或BNAOT（或对侧C3）中细胞的标记。共轭物在该系统中顺行运输，标记AOB到BNAOT、M和C3的传出投射。AOB至少接受2组同侧传入：一组来自C3，通过终纹，终止于内部颗粒层，第二组来自M和/或BNAOT，终止于IPL，可能在副嗅束中运行。
Antreograde and retrograde axonal tracing methods were combined with transection of the stria terminalis to investigate the centrifugal afferent connections of the accessory olfactory bulb in the mouse. Injection of 3H-proline into the postero-medial cortical amygdaloid nucleus (C3) gives rise to anterograde autoradiographic labeling of a pathway terminating in the internal granular layer of the accessory olfactory bulb (AOB). Transection of the ipsilateral stria terminalis completely abolishes labeling of this pathway. Injections further rostral, in the bed nucleus of the accessory olfactory tract (BNAOT) and medial amygdaloid nucleus (M), give rise to labeling of a 2nd ipsilateral afferent pathway to the AOB which terminates in the internal plexiform layer (IPL) and is unaffected by strial transection. Injections of wheat germ lectin-HRP [horseradish peroxidase] conjugate into the AOB confirm that it receives afferents from the ipsilateral BR AOT, M and C3 and from a few cells in the contralateral C3. Transection of the ipsilateral stria terminals prevents retrograde labeling of any cells in the ipsilateral C3, but does not affect labeling of cells in M or BNAOT (or contralateral C3). The conjugate is transported anterogradely in this system, labeling the efferent projections of the AOB to BNAOT, M and C3. The AOB receives at least 2 sets of ipsilateral afferents: one set from C3, via the stria terminalis, terminating in the internal granular layer, and a 2nd set from M and/or BNAOT terminating in the IPL and probably running in the accessory olfactory tract.