Effects of nonylphenol and ethinylestradiol on copper redhorse (Moxostoma hubbsi), an endangered species
Effects of nonylphenol and ethinylestradiol on copper redhorse (Moxostoma hubbsi), an endangered species
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DOI：
10.1016/j.ecoenv.2014.07.004
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发表时间：
2014-10-01
影响因子：
6.8
通讯作者：
Roy, Robert L.
中科院分区：
文献类型：
--
作者：
Maltais, Domynick;Roy, Robert L.
铜红马（Moxostoma hubbsi）是魁北克特有的濒危物种。其栖息地中污染物的存在，特别是内分泌干扰化学物质（EDC）已被认为是该物种遇到繁殖困难的部分原因。在本研究中，未成熟的铜红马在流通系统中暴露于雌激素表面活性剂壬基酚（NP；1、10和50μg/l）和合成雌激素17α-炔雌醇（EE2；10ng/l）21天。研究的终点包括一般健康指标（肝体指数和血细胞比容）、甲状腺激素、性类固醇、脑芳香酶活性、血浆和粘液卵黄蛋白原（VTG）、细胞色素P4501A蛋白表达和乙氧基试卤灵-O-脱乙基酶活性、热休克蛋白70（HSP70）和肌肉乙酰胆碱酯酶。暴露于 10 ng EE2/I 显着增加大脑芳香酶活性。暴露于 50 μg NP/l 导致血浆睾酮浓度显着降低，并显着诱导肝脏 HSP70 蛋白表达。 50 μg/l 的 NP 也诱导血浆和粘液 VTG。暴露于 NP 的未成熟铜红马表面粘液中 VTG 水平升高，及其与血浆 VTG 的相关性，支持使用粘液 VTG 作为非侵入性生物标志物来评估铜红马暴露于环境中的 EDC，并有助于该物种的恢复工作。本研究的结果表明，暴露于高环境相关浓度的 NP 和 EE2 会影响与铜红马繁殖相关的分子终点。皇冠版权所有 (C) 2014 由 Elsevier Inc. 出版。保留所有权利。
The copper redhorse, Moxostoma hubbsi, is an endangered species endemic to Quebec. The presence of contaminants, in particular endocrine disrupting chemicals (EDCs), in its habitat has been advanced as partly responsible for the reproductive difficulties encountered by the species. In the present study, immature copper redhorse were exposed to the estrogenic surfactant nonylphenol (NP; 1, 10 and 50 mu g/l) and the synthetic estrogen 17 alpha-ethinylestradiol (EE2; 10 ng/l) for 21 days in a flow-through system. The endpoints investigated included general health indicators (hepatosomatic index and hematocrit), thyroid hormones, sex steroids, brain aromatase activity, plasma and mucus vitellogenin (VTG), cytochrome P4501A protein expression and ethoxyresorufin-O-deethylase activity, heat shock protein 70 (HSP70) and muscle acetylcholinesterase. Exposure to 10 ng EE2/I significantly increased brain aromatase activity. Exposure to 50 mu g NP/l resulted in a significant reduction of plasma testosterone concentrations and a significant induction of hepatic HSP70 protein expression. NP at 50 mu g/l also induced plasma and mucus VTG. The presence of elevated VTG levels in the surface mucus of immature copper redhorse exposed to NP, and its correlation to plasma VTG, supports the use of mucus VTG as a non-invasive biomarker to evaluate copper redhorse exposure to EDCs in the environment and contribute to restoration efforts of the species. The results of the present study indicate that exposure to high environmentally relevant concentrations of NP and EE2 can affect molecular endpoints related to reproduction in the copper redhorse. Crown Copyright (C) 2014 Published by Elsevier Inc. All rights reserved.