Vacuolar and cytoskeletal dynamics during elicitor-induced programmed cell death in tobacco BY-2 cells
Vacuolar and cytoskeletal dynamics during elicitor-induced programmed cell death in tobacco BY-2 cells
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DOI：
10.4161/psb.3.9.6431
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Kuchitsu, Kazuyuki
中科院分区：
文献类型：
--
作者：
Higaki, Takumi;Kadota, Yasuhiro;Kuchitsu, Kazuyuki
植物细胞对环境胁迫的响应通常涉及到细胞形态的变化、细胞器和细胞骨架网络的分化和重新分布。烟草BY-2细胞为这些细胞内事件的体内成像提供了极好的模型系统。处理细胞周期同步的BY-2细胞与蛋白质卵菌诱导子，隐地蛋白，诱导高度同步的程序性细胞死亡（PCD），并提供了一个模型系统，以表征空泡和细胞骨架的动态过程中的PCD。连续观察发现动态重组的空泡和肌动蛋白微丝在执行过程中的PCD。我们进一步表征了隐地蛋白对细胞周期同步细胞中有丝分裂微管组织的影响。隐地蛋白治疗在S期抑制形成的preprophase带，皮质微管带，预测细胞分裂的网站。皮质微管保持其随机取向，直到它们在隐地蛋白处理后12小时的PCD执行期间逐渐发生破坏。对细胞器和细胞骨架网络的动态行为在致病信号诱导的PCD中可能的分子机制和生理作用进行了讨论。
Responses of plant cells to environmental stresses often involve morphological changes, differentiation and redistribution of various organelles and cytoskeletal network. Tobacco BY-2 cells provide excellent model system for in vivo imaging of these intracellular events. Treatment of the cell cycle-synchronized BY-2 cells with a proteinaceous oomycete elicitor, cryptogein, induces highly synchronous programmed cell death (PCD) and provide a model system to characterize vacuolar and cytoskeletal dynamics during the PCD. Sequential observation revealed dynamic reorganization of the vacuole and actin microfilaments during the execution of the PCD. We further characterized the effects cryptogein on mitotic microtubule organization in cell cycle-synchronized cells. Cryptogein treatment at S phase inhibited formation of the preprophase band, a cortical microtubule band that predicts the cell division site. Cortical microtubules kept their random orientation till their disruption that gradually occurred during the execution of the PCD twelve hours after the cryptogein treatment. Possible molecular mechanisms and physiological roles of the dynamic behavior of the organelles and cytoskeletal network in the pathogenic signal-induced PCD are discussed.