Chlorobaculum tepidum TLS displays a complex transcriptional response to sulfide addition.
Chlorobaculum tepidum TLS displays a complex transcriptional response to sulfide addition.
复制标题
温热绿杆菌 TLS 对硫化物添加表现出复杂的转录反应。
DOI：
10.1128/jb.01342-12
复制
发表时间：
2013
影响因子：
3.2
通讯作者：
Hanson,ThomasE
中科院分区：
文献类型：
--
作者：
Eddie,BrianJ;Hanson,ThomasE
暗绿杆菌是一种绿色硫细菌，是光养硫氧化的模式系统。尽管有超过20年的研究，我们对其电子供体代谢和调控的理解存在明显的差距。RNA测序（RNA-seq）用于提供C.在硫代硫酸盐作为唯一电子供体的生长过程中以及在向这种培养物中加入硫化物后的时间点，在硫化物添加后，121至150个蛋白质编码基因显示出随时间点而显著的表达变化。这些变化包括硫代硫酸盐和元素硫氧化基因表达的快速下降。具有增加的表达的基因和基因位点包括CT 1087，其编码在高硫化物浓度下生长所需的硫化物：醌氧化还原酶;多硫化物还原酶样复合操纵子，psrABC（CT 0496至CT 0494）;以及，令人惊讶的是，涉及铁获取的一大簇基因。最后，在厌氧细菌和古细菌中作为盒保守的两个基因CT 1276和CT 1277显示出表达的强烈增加。CT 1277基因产物含有DNA结合结构域，表明其在硫化物依赖性基因表达变化中的作用。
Chlorobaculum tepidum is a green sulfur bacterium (GSB) that is a model system for phototrophic sulfur oxidation. Despite over 2 decades of research, conspicuous gaps exist in our understanding of its electron donor metabolism and regulation. RNA sequencing (RNA-seq) was used to provide a global picture of the C. tepidum transcriptome during growth on thiosulfate as the sole electron donor and at time points following the addition of sulfide to such a culture. Following sulfide addition, 121 to 150 protein-coding genes displayed significant changes in expression depending upon the time point. These changes included a rapid decrease in expression of thiosulfate and elemental sulfur oxidation genes. Genes and gene loci with increased expression included CT1087, encoding a sulfide:quinone oxidoreductase required for growth in high sulfide concentrations; a polysulfide reductase-like complex operon,psrABC(CT0496 to CT0494); and, surprisingly, a large cluster of genes involved in iron acquisition. Finally, two genes that are conserved as a cassette in anaerobic bacteria and archaea, CT1276 and CT1277, displayed a strong increase in expression. The CT1277 gene product contains a DNA-binding domain, suggesting a role for it in sulfide-dependent gene expression changes.