CHANGES IN HUMAN MUSCLE PROTEIN-SYNTHESIS AFTER RESISTANCE EXERCISE
CHANGES IN HUMAN MUSCLE PROTEIN-SYNTHESIS AFTER RESISTANCE EXERCISE
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DOI：
10.1152/jappl.1992.73.4.1383
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发表时间：
1992-10-01
影响因子：
3.3
通讯作者：
SMITH, K
中科院分区：
文献类型：
--
作者：
CHESLEY, A;MACDOUGALL, JD;SMITH, K
本研究的目的是探讨肌肉蛋白质合成（MPS）的变化幅度和时间过程后，单回合的抗阻运动。两组六名男性受试者进行重阻力运动与手肘屈肌的一只手臂，而另一只手臂作为控制。运动后4小时（A组）和24小时（B组），通过肌肉活检样品中L-[1-C-13]亮氨酸掺入量的增加，评估运动（ex）和对照（con）肱二头肌的MPS。此外，确定RNA容量和RNA活性以评估转录和/或翻译过程是否影响MPS。与两组对照组相比，实验组肱二头肌的MPS显著升高（A组，实验组0.1007 +/- 0.0330 vs.对照组0.067 +/-0.0204%/h; B组实验组0.0944 +/- 0.0363 vs.对照组0.0452 +/-0.0126%/h）。相对于对照组二头肌，两组前二头肌中的RNA容量没有变化，而两组前二头肌中的RNA活性显著升高（A组，前0.19 +/- 0.10对对照0.12 +/- 0.05 μ g蛋白。h-1。mug-1总RNA; B组，ex 0.18 +/- 0.06 vs. con 0.08 +/- 0.02 mug蛋白。h-1。μ g-1总RNA）。结果表明，一次大阻力运动可以增加肱二头肌MPS长达24小时后运动。此外，这些增加似乎是由于转录后事件的变化。
The purpose of this study was to investigate the magnitude and time course for changes in muscle protein synthesis (MPS) after a single bout of resistance exercise. Two groups of six male subjects performed heavy resistance exercise with the elbow flexors of one arm while the opposite arm served as a control. MPS from exercised (ex) and control (con) biceps brachii was assessed 4 (group A) and 24 h (group B) postexercise by the increment in L-[1-C-13]leucine incorporation into muscle biopsy samples. In addition, RNA capacity and RNA activity were determined to assess whether transcriptional and/or translational processes affected MPS. MPS was significantly elevated in biceps of the ex compared with the con arms of both groups (group A, ex 0.1007 +/- 0.0330 vs. con 0.067 +/-0.0204 %/h; group B ex 0.0944 +/- 0.0363 vs. con 0.0452 +/- 0.0126 %/h). RNA capacity was unchanged in the ex biceps of both groups relative to the con biceps, whereas RNA activity was significantly elevated in the ex biceps of both groups (group A, ex 0.19 +/- 0.10 vs. con 0.12 +/- 0.05 mug protein . h-1 . mug-1 total RNA; group B, ex 0.18 +/- 0.06 vs. con 0.08 +/- 0.02 mug protein . h-1 . mug-1 total RNA). The results indicate that a single bout of heavy resistance exercise can increase biceps MPS for up to 24 h postexercise. In addition, these increases appear to be due to changes in posttranscriptional events.