During cooled storage the extender influences processed autophagy marker light chain 3 (LC3B) of stallion spermatozoa
During cooled storage the extender influences processed autophagy marker light chain 3 (LC3B) of stallion spermatozoa
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DOI：
10.1016/j.anireprosci.2014.01.001
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发表时间：
2014-02-01
影响因子：
2.2
通讯作者：
Pena, F. J.
中科院分区：
文献类型：
--
作者：
Gallardo Bolanos, J. M.;Miro Moran, A.;Pena, F. J.
为了探讨自噬标记轻链3(LC3B)蛋白在种马精液冷冻保存过程中对精子存活率的影响，将分离的精液用两种不同的稀释液KMT和INRA 96进行稀释，并在5℃下孵育5天来评价LC3B的处理能力和精子质量。在孵育3天后，两种延长器的总活动率都有所下降，但在INRA96延伸器中，前进运动精子的百分比更高(P<；0.05)。在冷冻保存第5天，所有精子参数均显著下降，但与第3天相比，在INRA 96中扩展的样品保持不变的活力，而在KMT中扩展的样品与第3天的数据相比有进一步的下降。活精子百分率随着孵育时间的延长而下降，但仅在KMT孵化的样品中。该延伸剂对冷藏过程中的LC3B加工有明显的效果。在KMT稀释液中保存的精子没有发生LOB加工，而在INRA96中孵育的精子在冷藏5天后LOB加工增加(P<；0.01)。种马精子在冷藏过程中经历LC3B周转，但程度取决于所使用的稀释剂。显然，LC3B的周转与提高存活率有关。(C)2014爱思唯尔B.V.保留所有权利。
To investigate the role of the processed autophagy marker light chain 3 (LC3B) protein in sperm survival in stallion semen processing during cooled storage, split ejaculates were diluted in two different extenders, KMT and INRA 96, and LC3B processing and sperm quality evaluated during incubation at 5 degrees C for five days. After 3 days of incubation there was a drop in total motility in both extenders, although the percentage of progressive motile sperm was greater (P < 0.05) in samples extended in INRA96. On Day 5 of cooled storage all sperm parameters decreased significantly independent of the extender, however, samples extended in INRA 96 maintained motility values while those extended in KMT had a further decrease in motility compared with data collected on Day 3 of incubation. The percentage of live sperm decreased over the time of incubation, but only in samples incubated in KMT. The extender had a marked effect in LC3B processing during cooled storage. Spermatozoa maintained in KMT extender did not exhibit LOB processing, while in spermatozoa incubated in INRA96 there was an increase (P < 0.01) in LOB processing after 5 days of cooled storage. Stallion spermatozoa experience LC3B turnover during cooled storage, however, the extent depends on the extender used. Apparently LC3B turnover is associated with enhanced survival. (C) 2014 Elsevier B.V. All rights reserved.