PLASMODIUM-FALCIPARUM - STAGE-SPECIFIC LACTATE PRODUCTION IN SYNCHRONIZED CULTURES
PLASMODIUM-FALCIPARUM - STAGE-SPECIFIC LACTATE PRODUCTION IN SYNCHRONIZED CULTURES
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DOI：
10.1016/0014-4894(82)90048-0
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发表时间：
1982-01-01
影响因子：
2.1
通讯作者：
PARQUETTE, AR
中科院分区：
文献类型：
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作者：
PFALLER, MA;KROGSTAD, DJ;PARQUETTE, AR
用明胶进行顺序处理，然后用山梨醇产生的恶性疟原虫培养物进行处理，这些培养物对于 .gtoreq 保持同步。 144 小时。与寄生虫的其他无性红细胞阶段相比，裂殖体阶段产生更多的乳酸（11-21 vs. 4-7 neq/h-106 寄生红细胞 [PRBC]）。异步培养物中的乳酸产量相对稳定在 8-11 neq/h-106 PRBC，并且与寄生虫血症成正比（P < 0.001）。
Sequential treatment with gelatin followed by sorbitol-produced P. falciparum cultures that remained synchronous for .gtoreq. 144 h. The schizont stage was associated with more lactate production than the other asexual erythrocytic stages of the parasite (11-21 vs. 4-7 neq/h-106 parasitized red blood cells [PRBC]). Lactate production in asynchronous cultures was relatively constant at 8-11 neq/h-106 PRBC and was directly proportional to parasitemia (P < 0.001).