Detection and differentiation of Plasmodium species by polymerase chain reaction and colorimetric detection in blood samples of patients with suspected malaria
Detection and differentiation of Plasmodium species by polymerase chain reaction and colorimetric detection in blood samples of patients with suspected malaria
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DOI：
10.1016/j.diagmicrobio.2003.10.014
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发表时间：
2004-05-01
影响因子：
2.9
通讯作者：
Sloots, TP
中科院分区：
文献类型：
--
作者：
Whiley, DM;LeCornec, GM;Sloots, TP
聚合酶链式反应（PCR）现已被认为是检测血液中疟原虫种类的灵敏且特异的方法。在这项研究中。我们利用物种特异性比色检测进行 PCR 检测，测试了来自疑似疟疾患者的 279 份血液样本。并将结果与光学显微镜进行比较。总体而言，279 个样本中的 270 个样本的两种检测结果一致。在 131 份 (47.0%) 样本中检测到间日疟原虫。 64 件（22.9%）标本中出现恶性疟原虫，6 件（2.1%）标本中出现卵形疟原虫，5 件（1.8%）标本中出现三日疟原虫。在另外 10 份 (3.6%) 样本中检测到恶性疟原虫和间日疟原虫，其中 54 份 (19.3%) 样本两次检测均呈阴性。在剩下的九个样本中，显微镜要么未能检测到寄生虫，要么错误地识别了存在的物种。总之，PCR 检测的灵敏度、特异性和简单性使其特别适合在诊断实验室中使用。 (C) 2004 Elsevier Inc. 保留所有权利。
Polymerase chain reaction (PCR) is now recognized as a sensitive and specific method for detecting Plasmodium species in blood. In this Study. we tested 279 blood samples, from patients with Suspected malaria, by a PCR assay utilizing species-specific colorimetric detection. and compared the results to light microscopy. Overall, both assays were in agreement for 270 of the 279 specimens. P. vivax was detected in 131 (47.0%) specimens. P. falciparum in 64 (22.9%) specimens, P. ovale in 6 (2.1%) specimens, and P. malariae in 5 (1.8%) specimens. Both P. falciparum and P. vivax were detected in a further 10 (3.6%) specimens, and 54 (19.3%) specimens were negative by both assays. In the remaining nine specimens, microscopy either failed to detect the parasite or incorrectly identified the species present. In summary, the sensitivity, specificity and simplicity of the PCR assay makes it particularly suitable for use in a diagnostic laboratory. (C) 2004 Elsevier Inc. All rights reserved.