Nucleotide variation at the Gpdh locus in the genus Drosophila.
Nucleotide variation at the Gpdh locus in the genus Drosophila.
复制标题
果蝇属 Gpdh 基因座的核苷酸变异。
DOI：
10.1093/genetics/143.1.375
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发表时间：
1996
期刊：
影响因子：
3.3
通讯作者：
Wells,RS
中科院分区：
文献类型：
--
作者：
Wells,RS
Gpdh基因座在大范围的果蝇物种中被测序。与氨基酸水平上的极端进化限制相反，同义位点的进化速度与其他基因相当。Gpdh核苷酸序列被用来推断系统发育树，并详细研究了该属物种之间的关系。对D. pseudobscuralealed没有氨基酸变异。应用改良的McDonald-Kreitman检验，obscuragroup中物种之间的氨基酸差异似乎并不过分，这意味着漂移足以解释该位点的氨基酸变化模式。此外，Hudson-Kreitman-Aguadé检验表明，拟暗纹果蝇Gpdh基因座的多态性水平与其它基因座的多态性水平相当。因此，在theGpdh基因座的物种内和物种间的核苷酸变异模式与中性模型一致。
TheGpdhlocus was sequenced in a broad range ofDrosophilaspecies. In contrast to the extreme evolutionary constraint seen at the amino acid level, the synonymous sites evolve at rates comparable to those of other genes.Gpdhnucleotide sequences were used to infer a phylogenetic tree, and the relationships among the species of theobscuragroup were examined in detail. A survey of nucleotide polymorphism withinD. pseudoobscurarevealed no amino acid variation in this species. Applying a modified McDonald-Kreitman test, the amino acid divergence between species in theobscuragroup does not appear to be excessive, implying that drift is adequate to explain the patterns of amino acid change at this locus. In addition, the level of polymorphism at theGpdhlocus inD. pseudoobscurais comparable to that found at other loci, as determined by a Hudson-Kreitman-Aguadé test. Thus, the pattern of nucleotide variation within and between species at theGpdhlocus is consistent with a neutral model.