Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study.
Serum fatty acid-binding protein 4 (FABP4) concentration is associated with insulin resistance in peripheral tissues, A clinical study.
复制标题
DOI：
10.1371/journal.pone.0179737
复制
发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Yamamoto K
中科院分区：
文献类型：
--
作者：
Nakamura R;Okura T;Fujioka Y;Sumi K;Matsuzawa K;Izawa S;Ueta E;Kato M;Taniguchi SI;Yamamoto K
2型糖尿病（T2 DM）是由胰岛素抵抗和β细胞功能障碍引起的。近年来的研究报道，骨骼肌中与胰岛素敏感性相关的几种标志物脂联素和其他参数如脂肪酸结合蛋白（FABP 4）等均被报道调节胰岛素抵抗，但目前尚不清楚哪种因素最主要影响T2 DM的胰岛素抵抗。在这项横断面研究中，我们评估了几种生物标志物与T2 DM和健康对照者的胰岛素抵抗和胰岛素分泌的关系。我们招募了30名参与者（12名T2 DM和18名非糖尿病健康对照）。参与者进行了一次膳食耐量试验，在此期间，血浆葡萄糖，胰岛素和血清C肽免疫反应性进行了测量。我们进行了高胰岛素-正葡萄糖钳夹，并测量了葡萄糖处置率（GDR）。采用ELISA法检测空腹血清脂联素、胰岛素样生长因子-1、鸢尾素、自分泌运动因子、FABP 4和白细胞介素-6水平。我们发现T2 DM中FABP 4浓度与GDR之间存在强负相关性（r =-0.657，p = 0.020）。FABP 4还与T2 DM膳食耐量试验期间的胰岛素分泌呈正相关（IRI（120）：r = 0.604，p = 0.038），并与非DM受试者的胰岛素生成指数呈正相关（r = 0.536，p = 0.022）。Autotaxin也与GDR有关。但与胰岛素分泌无关。结果表明，2型糖尿病患者血清FABP 4水平与胰岛素抵抗和胰岛素分泌有关。这表明FABP 4可能在葡萄糖稳态中起重要作用。
Type 2 diabetes mellitus (T2DM) is caused by insulin resistance and β cell dysfunction. In recent studies reported that several markers associated with insulin sensitivity in skeletal muscle, Adiponectin and other parameters, such as fatty acid-binding protein (FABP4), have been reported to regulate insulin resistance, but it remains unclear which factor mostly affects insulin resistance in T2DM. In this cross-sectional study, we evaluated the relationships between several kinds of biomarkers and insulin resistance, and insulin secretion in T2DM and healthy controls. We recruited 30 participants (12 T2DM and 18 non-diabetic healthy controls). Participants underwent a meal tolerance test during which plasma glucose, insulin and serum C-peptide immunoreactivity were measured. We performed a hyperinsulinemic-euglycemic clamp and measured the glucose-disposal rate (GDR). The fasting serum levels of adiponectin, insulin-like growth factor-1, irisin, autotaxin, FABP4 and interleukin-6 were measured by ELISA. We found a strong negative correlation between FABP4 concentration and GDR in T2DM (r = -0.657, p = 0.020). FABP4 also was positively correlated with insulin secretion during the meal tolerance test in T2DM (IRI (120): r = 0.604, p = 0.038) and was positively related to the insulinogenic index in non-DM subjects (r = 0.536, p = 0.022). Autotaxin was also related to GDR. However, there was no relationship with insulin secretion. We found that serum FABP4 concentration were associated with insulin resistance and secretion in T2DM. This suggests that FABP4 may play an important role in glucose homeostasis.