Listeria monocytogenes serotype identification by PCR
Listeria monocytogenes serotype identification by PCR
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DOI：
10.1128/jcm.41.12.5537-5540.2003
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发表时间：
2003-12-01
影响因子：
9.4
通讯作者：
Call, DR
中科院分区：
文献类型：
--
作者：
Borucki, MK;Call, DR
血清分型是普遍接受的单核细胞增生李斯特菌亚型分型方法。菌株血清型的鉴定可以区分重要的食源性菌株（1/2a、1/2b 和 4b），并为比较不同实验室和不同技术分析的分离株提供“金标准”。尽管最近描述了一种有效的酶联免疫吸附测定血清分型方案，但 PCR 血清分型引物的鉴定将进一步增加该分类系统的简便性和可访问性。血清分型 PCR 引物是根据单核细胞增生利斯特氏菌基因组的可变区设计的。三个引物组与先前描述的 III 区引物组结合使用，以便将 122 个单核细胞增生李斯特氏菌菌株分为五个血清型组 [1/2a(3a)、1/2b、1/2c(3c)、4b(d,e) 和 4a/c]。对于属于血清型1/2a、1/2b、1/2c和4b的菌株，PCR方法的结果与常规玻片凝集法的结果分别为97%、100%、94%和91%。
Serotyping is a universally accepted subtyping method for Listeria monocytogenes. Identification of the strain serotype permits differentiation between important food-borne strains (1/2a, 1/2b, and 4b) and provides a "gold standard" for comparing isolates analyzed in different labs and with different techniques. Although an efficient enzyme-linked immunosorbent assay serotyping protocol was described recently, identification of PCR serotyping primers would further increase the ease and accessibility of this classification system. Serotyping PCR primers were designed from variable regions of the L. monocytogenes genome. Three primer sets were used in conjunction with a previously described Division III primer set in order to classify 122 L. monocytogenes strains into five serotype groups [1/2a(3a), 1/2b, 1/2c(3c), 4b(d,e), and 4a/c]. Results of the PCR method agreed with those of the conventional slide agglutination method for 97, 100, 94, and 91% of strains belonging to serotypes 1/2a, 1/2b, 1/2c, and 4b, respectively.