Genetic architecture of growth traits in Populus revealed by integrated quantitative trait locus (QTL) analysis and association studies
Genetic architecture of growth traits in Populus revealed by integrated quantitative trait locus (QTL) analysis and association studies
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通过综合数量性状位点（QTL）分析和关联研究揭示杨属生长性状的遗传结构
DOI：
10.1111/nph.13695
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发表时间：
2016-02-01
期刊：
影响因子：
9.4
通讯作者：
Zhang, Deqiang
中科院分区：
文献类型：
--
作者：
Du, Qingzhang;Gong, Chenrui;Zhang, Deqiang
破译多年生植物多基因性状的遗传结构可以为分子标记辅助育种提供信息。本研究采用数量性状位点（QTL）分析、高分辨率连锁图和多基因关联图相结合的方法，对杨树的遗传结构进行了研究杨属连锁群体生长性状潜在QTL（加性、显性和上位性效应）分析（1200个后代）和自然种群（435个个体）。树高、胸高直径和树干体积的17个QTL映射到11个连锁群（比值对数（LOD）>= 2.5），解释表型变异的2.7-18.5%。通过比较定位和转录组分析，从13个QTL的片段同源区（SHRs）中筛选出187个表达基因（10 046个SNP）。利用多基因关联模型，在10个QTL的63个有希望的基因中检测到202个显著的SNP位点（P
Deciphering the genetic architecture underlying polygenic traits in perennial species can inform molecular marker-assisted breeding. Recent advances in high-throughput sequencing have enabled strategies that integrate linkage-linkage disequilibrium (LD) mapping in Populus.We used an integrated method of quantitative trait locus (QTL) dissection with a high-resolution linkage map and multi-gene association mapping to decipher the nature of genetic architecture (additive, dominant, and epistatic effects) of potential QTLs for growth traits in a Populus linkage population (1200 progeny) and a natural population (435 individuals).Seventeen QTLs for tree height, diameter at breast height, and stem volume mapped to 11 linkage groups (logarithm of odds (LOD) >= 2.5), and explained 2.7-18.5% of the phenotypic variance. After comparative mapping and transcriptome analysis, 187 expressed genes (10 046 common single nucleotide polymorphisms (SNPs)) were selected from the segmental homology regions (SHRs) of 13 QTLs. Using multi-gene association models, we observed 202 significant SNPs in 63 promising genes from 10 QTLs (P