Real time detection of antibody-antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system
Real time detection of antibody-antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system
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DOI：
10.1088/1674-1056/21/2/020601
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发表时间：
2012-02
影响因子：
1.7
通讯作者：
Hong-Yan 洪艳 Zhang 张;Li-Quan 立泉 Yang 杨;Lan 岚 Meng 孟;J. Nie 聂;Ting-Yin 廷银 Ning 宁;W. Liu 刘;Jia-Yu 嘉宇 Sun 孙;Peng-Fei 鹏飞 Wang 汪
中科院分区：
文献类型：
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作者：
Hong-Yan 洪艳 Zhang 张;Li-Quan 立泉 Yang 杨;Lan 岚 Meng 孟;J. Nie 聂;Ting-Yin 廷银 Ning 宁;W. Liu 刘;Jia-Yu 嘉宇 Sun 孙;Peng-Fei 鹏飞 Wang 汪
A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science.
