Composition and Structure of Single Cell Oil Produced by Schizochytrium limacinum SR31
Composition and Structure of Single Cell Oil Produced by Schizochytrium limacinum SR31
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裂殖壶菌SR31产生的单细胞油的组成和结构
DOI：
10.1007/s11746-016-2881-y
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发表时间：
2016-10-01
影响因子：
2
通讯作者：
Wang, Xingguo
中科院分区：
文献类型：
--
作者：
Zhang, Tao;Lou, Fei;Wang, Xingguo
裂殖壶菌limacinum sp.在商业上用于生产单细胞油（SCO），但其详细的脂质分布仍然未知。为了分析裂殖壶菌Schizochytriumlimacinum SR 31产生的SCO的组成和结构，采用硅胶柱层析法将稳定生长期的SCO分离为中性脂、极性脂和糖脂。脂质分类结果显示，甘油三酯（TAG）是主要的甘油酯（93.81%）.通过气相色谱-质谱（GC-MS）分析脂肪酸（FA），证明棕榈酸（29.78%）和二十二碳六烯酸（25.64%）是主要的FA。采用电喷雾四极杆飞行时间质谱联用超高效液相色谱研究了TAG的结构。鉴定出80多个物种。FA在TAG中的位置分布可能有助于进一步研究脂肪转换阶段FA的变化和SCO的营养评价。
Schizochytrium limacinum sp. was commercially utilized to produce single cell oil (SCO), but its detailed lipid profile remains unknown. In order to analyze the composition and structure of SCO produced by Schizochytrium limacinum SR31, the SCO extracted at stationary growth phase was separated into neutral lipids (NL), polar lipids and glycolipids by using silica gel column chromatography. The result of lipid classes in NL revealed that triacylglycerol (TAG) was the primary glyceride (93.81 %). Fatty acid (FA) analysis by gas chromatography-mass spectrometer (GC-MS) proved that palmitic acid (29.78 %) and docosahexaenoic acid (25.64 %) were the major FA. Quadrupole time of flight mass spectrometry in electrospray ionization mode coupled with ultra-performance liquid chromatography was carried out to investigate TAG structures. More than 80 species were identified. Positional distribution of FA in TAG might contribute to further study of the FA shifts during the lipid turnover stage and nutritional evaluation of the SCO.