Label-free fluorescence lifetime spectroscopy detects radiation-induced necrotic changes in live brain in real-time
Label-free fluorescence lifetime spectroscopy detects radiation-induced necrotic changes in live brain in real-time
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DOI：
10.1364/boe.9.003559
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发表时间：
2018-08-01
影响因子：
3.4
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Hartl, Brad A.;Ma, Htet S. W.;Marcu, Laura
目前的临床成像模式不能可靠地识别放射治疗后坏死的脑组织区域。这给立体定位活检和手术决策带来了挑战。时间分辨荧光光谱（TRFS）提供了一种快速识别坏死组织的方法，通过其独特的自发荧光特征，导致组织分解和辐射损伤区域的代谢谱改变。在放射性坏死的活体动物模型中进行的研究表明，与健康组织相比，坏死组织的特征在于平均寿命和氧化还原率分别增加27%和108%。此外，TRFS检测到MRI不可见但经组织病理学证实的辐射损伤组织。目前的结果表明，TRFS能够实时识别辐射损伤的脑组织，并表明其有潜力协助手术指导和MRI引导活检程序。(C)根据OSA开放获取出版协议的条款，2018年美国光学学会
Current clinical imaging modalities do not reliably identify brain tissue regions with necrosis following radiotherapy. This creates challenges for stereotaxic biopsies and surgical decision making. Time-resolved fluorescence spectroscopy (TRFS) provides a means to rapidly identify necrotic tissue by its distinct autofluorescence signature resulting from tissue breakdown and altered metabolic profiles in regions with radiation damage. Studies conducted in a live animal model of radiation necrosis demonstrated that necrotic tissue is characterized by respective increases of 27% and 108% in average lifetime and redox ratio, when compared with healthy tissue. Moreover, radiation-damaged tissue not visible by MRI but confirmed by histopathology, was detected by TRFS. Current results demonstrate the ability of TRFS to identify radiation-damaged brain tissue in real-time and indicates its potential to assist with surgical guidance and MRI-guided biopsy procedures. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement