Quantitative proteomic study of human prostate cancer cells with different metastatic potentials
Quantitative proteomic study of human prostate cancer cells with different metastatic potentials
复制标题
不同转移潜能人前列腺癌细胞的定量蛋白质组学研究
DOI：
10.3892/ijo.2016.3378
复制
发表时间：
2016-04-01
影响因子：
5.2
通讯作者：
Li, Yulin
中科院分区：
文献类型：
--
作者：
Li, Qun;Li, Yilei;Li, Yulin
转移性播散是包括前列腺癌（PCa）在内的大多数癌症的特征，并且是治疗失败和死亡的主要原因。本研究的目的是探讨前列腺癌转移的机制，并寻找潜在的预后指标，利用蛋白质组学。二维荧光差异凝胶电泳（2D-DIGE）用于定量蛋白质在正常前列腺上皮细胞，骨转移衍生的PC-3细胞，和内脏转移衍生的PC-3 M细胞。通过流式细胞术、电子显微镜、增殖细胞核抗原测定和伤口愈合测定证实转移潜力。比较具有不同转移潜能的PCa细胞（LNcap、DU 145、PC-3和PC-3 M）与正常前列腺上皮细胞（RWPE-1）之间的差异蛋白表达。使用果阿、UniProt和GeneCards分析在人前列腺组织中选择的候选蛋白。86个蛋白质在细胞系间差异表达（>1.5倍，P
Metastatic dissemination is a feature of most cancers including prostate cancer (PCa), and is the main cause of treatment failure and mortality. The aim of the study is to explore the mechanisms of PCa metastasis and to search for potential prognostic markers using proteomics. Two-dimensional fluorescent differential gel electrophoresis (2D-DIGE) was used to quantify proteins in normal prostate epithelial cells, bone metastasis-derived PC-3 cells, and visceral metastasis-derived PC-3M cells. Metastatic potential was confirmed by flow cytometry, electron microscopy, proliferating cell nuclear antigen assay, and wound healing assay. Differential protein expression was compared between PCa cells with different metastatic potentials (LNcap, DU145, PC-3 and PC-3M) and normal prostate epithelial cells (RWPE-1). Selected candidate proteins in human prostate tissues were analyzed using GOA, UniProt and GeneCards analyses. Eighty-six proteins were differentially expressed between cell lines (>1.5-fold, P