Live-cell 3D super-resolution imaging in thick biological samples
Live-cell 3D super-resolution imaging in thick biological samples
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DOI：
10.1038/nmeth.1744
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发表时间：
2011-12-01
期刊：
影响因子：
48
通讯作者：
Diaspro, Alberto
中科院分区：
文献类型：
--
作者：
Zanacchi, Francesca Cella;Lavagnino, Zeno;Diaspro, Alberto
我们展示了三维（3D）超分辨率活细胞成像通过厚标本（50-150 μ m），耦合远场单个分子定位与选择性平面照明显微镜（SPIM）。选择性平面照明的改进信噪比允许在厚散射标本中进行单分子的纳米定位，而无需激活或激发焦平面外的分子。我们报告了细胞球体的三维超分辨率成像。
We demonstrate three-dimensional (3D) super-resolution live-cell imaging through thick specimens (50-150 mu m), by coupling far-field individual molecule localization with selective plane illumination microscopy (SPIM). The improved signal-to-noise ratio of selective plane illumination allows nanometric localization of single molecules in thick scattering specimens without activating or exciting molecules outside the focal plane. We report 3D super-resolution imaging of cellular spheroids.