Measurement of plasma testosterone by means of competitive protein binding analysis.
Measurement of plasma testosterone by means of competitive protein binding analysis.
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通过竞争性蛋白结合分析测量血浆睾酮。
DOI：
10.1210/jcem-29-6-854
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发表时间：
1969
期刊：
影响因子：
--
通讯作者：
A. Bongiovanni
中科院分区：
文献类型：
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作者：
R. Rosenfield;W. Eberlein;A. Bongiovanni
本文介绍了竞争蛋白结合分析测定血浆睾酮(T)的方法。在分析之前，用单一薄层层析步骤纯化5.0ml血浆的乙醚提取物。用1：10 0稀释的妊娠晚期血浆在4℃下反应，用木炭在葡聚糖中的粘性悬浮液分离结合T和游离T，测定T具有较高的准确度(b=0.963，r=0.919)和灵敏度(0.2 5~2.5 ng/样品)。精密度(15%)和特异度适合临床使用。正常女性平均血浆T为46.5±19.3 ng/100ml(32.2-70.3)，正常男性为533±259 ng/100ml(238-1001)。
ABSTRACT A method for the measurement of plasma testosterone (T) by competitive protein binding analysis is described. An ether extract of 5.0 ml plasma is purified by a single thin layer chromatographic step prior to analysis. Third trimester pregnancy plasma diluted 1:100 is reacted at 4 C, and a viscous suspension of charcoal in dextran is utilized to separate bound from free T. T is measured with high accuracy (b=0.963, r =0.919) and sensitivity (0.25–2.5 ng/sample). Precision (15%) and specificity are suitable for clinical use. The mean plasma T found in normal females is 46.5 ±19.3 ng/100 ml (range 32.2–70.3) and in normal males 533±259 ng/100 ml (range 238–1001).