Stem cell attachment to layer-by-layer assembled TiO2 nanoparticle thin films
Stem cell attachment to layer-by-layer assembled TiO2 nanoparticle thin films
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DOI：
10.1016/j.biomaterials.2006.03.042
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发表时间：
2006-08-01
期刊：
影响因子：
14
通讯作者：
Mills, David K.
中科院分区：
文献类型：
--
作者：
Kommireddy, Dinesh S.;Sriram, Shashikanth M.;Mills, David K.
表面形貌是影响细胞贴壁和铺展的重要因素之一。在本研究中，选择逐层组装的二氧化钛（TiO 2）纳米颗粒薄膜用于小鼠间充质干细胞（MSC）的附着、增殖和扩散研究。增加的表面粗糙度，观察到与逐层组装的TiO 2薄膜的数量增加。四层TiO 2薄膜显示出比单层薄膜和对照表面更高的附着细胞数量。从细胞毒性试验中观察到，在TiO 2涂覆的基质上培养后，MSC没有经历细胞毒性作用。用扫描电子显微镜观察的细胞铺展显示在粗糙表面上铺展的速率更快。在12 h时，四层基质上的细胞显示出完全铺展，而在24 h时，对照表面和单层表面上的大多数细胞保持圆形形态。总之，TiO2纳米颗粒薄膜成功地与聚电解质交替组装，MSC的体外研究表明，细胞在粗糙表面上的附着和更快的扩散增加。(c)2006爱思唯尔有限公司版权所有。
Surface topography is one of the most important factors influencing the attachment and spreading of cells. In the present study, layer-by-layer assembled titanium dioxide (TiO2) nanoparticle thin films were chosen for attachment, proliferation and spreading studies on mouse mesenchymal stem cells (MSC). Increasing surface roughness was observed with increasing number of layer-by-layer assembled TiO2 thin films. Four layer TiO2 thin film showed higher number of attached cells than a one layer thin film and control surfaces. MSCs experienced no cytotoxic effects after culture on the TiO2 coated substrates as observed from the cytotoxicity tests. Cell spreading, visualized with scanning electron microscopy, showed a faster rate of spreading on a rougher surface. Cells on a four-layer substrate, at 12 h showed complete spreading, where as most of the cells on a control surface and a one-layer surface, at 24 h, retained a rounded morphology. In conclusion, TiO2 nanoparticle thin films were successfully assembled in alternation with polyelectrolytes and in-vitro studies with MSC showed an increase in the attachment and faster spreading of cells on rougher surfaces. (c) 2006 Elsevier Ltd. All rights reserved.