Ultrafast Polymer Dynamics through a Nanopore
Ultrafast Polymer Dynamics through a Nanopore
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DOI：
10.1021/acs.nanolett.2c03546
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发表时间：
2022-10-31
期刊：
影响因子：
10.8
通讯作者：
Drndic, Marija
中科院分区：
文献类型：
--
作者：
Lin, Chih-Yuan;Fotis, Riley;Drndic, Marija
超薄纳米孔传感器能够以亚微秒级的时间分辨率对单分子和聚合物进行测量，这得益于高电流信号（约10 - 30纳安）。我们首次展示了在10兆赫兹带宽下对双链DNA（dsDNA）超快易位和折叠动力学的实验性探测，每25纳秒采集一个数据点（150兆字节/秒）。通过引入一种严谨的算法，我们能够准确识别易位事件中出现的每个电流水平，并阐明动态的折叠和展开行为。该系统出色的灵敏度揭示了在较低带宽下短暂折叠状态的变形。这项工作重新审视了将双链DNA作为一种模型聚合物的探测，并开发了广泛适用的方法。传感器信号、仪器设备以及大数据分析方法的综合改进，以前所未有的小时间尺度揭示了生物分子动力学。
Ultrathin nanopore sensors allow single-molecule and polymer measurements at sub-microsecond time resolution enabled by high current signals (similar to 10-30 nA). We demonstrate for the first time the experimental probing of the ultrafast translocation and folded dynamics of double-stranded DNA (dsDNA) through a nanopore at 10 MHz bandwidth with acquisition of data points per 25 ns (150 MB/s). By introducing a rigorous algorithm, we are able to accurately identify each current level present within translocation events and elucidate the dynamic folded and unfolded behaviors. The remarkable sensitivity of this system reveals distortions of short-lived folded states at a lower bandwidth. This work revisits probing of dsDNA as a model polymer and develops broadly applicable methods. The combined improvements in sensor signals, instrumentation, and large data analysis methods uncover biomolecular dynamics at unprecedentedly small time scales.