Nanog Regulates Proliferation During Early Fish Development
Nanog Regulates Proliferation During Early Fish Development
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DOI：
10.1002/stem.133
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发表时间：
2009-01-01
期刊：
影响因子：
5.2
通讯作者：
Mullor, Jose L.
中科院分区：
文献类型：
--
作者：
Camp, Esther;Sanchez-Sanchez, Ana V.;Mullor, Jose L.
Nanog参与控制体外干细胞的多能性和分化。然而，其在体内的功能仅在小鼠胚胎中进行了研究，并且各种报告表明Nanog可能不需要调节分化。为了更好地了解内源性Nanog功能，应该引入更多的动物模型来补充小鼠模型。在这里，我们鉴定了硬骨鱼中哺乳动物Nanog基因的同源物，并描述了青鳉（Oryzias latipes）胚胎发育期间和成年性腺中Ol-Nanog mRNA和蛋白质的内源表达。使用青鳉鱼作为脊椎动物模型来研究Nanog功能，我们证明了Ol-Nanog对于发育中胚胎的S-相变和增殖是必需的。此外，抑制或过表达的Ol-Nanog不影响基因表达的各种多能性和分化标志物，这表明这种转录因子可能不发挥直接作用，在胚胎胚层分化。干细胞2009; 27：2081-2091
Nanog is involved in controlling pluripotency and differentiation of stem cells in vitro. However, its function in vivo has been studied only in mouse embryos and various reports suggest that Nanog may not be required for the regulation of differentiation. To better understand endogenous Nanog function, more animal models should be introduced to complement the murine model. Here, we have identified the homolog of the mammalian Nanog gene in teleost fish and describe the endogenous expression of Ol-Nanog mRNA and protein during medaka (Oryzias latipes) embryonic development and in the adult gonads. Using medaka fish as a vertebrate model to study Nanog function, we demonstrate that Ol-Nanog is necessary for S-phase transition and proliferation in the developing embryo. Moreover, inhibition or overexpression of Ol-Nanog does not affect gene expression of various pluripotency and differentiation markers, suggesting that this transcription factor may not play a direct role in embryonic germ layer differentiation. STEM CELLS 2009; 27: 2081-2091