Polymorphism of the 3' open reading frame of the virus associated with the acquired immune deficiency syndrome, human T-lymphotropic virus type III.
Polymorphism of the 3' open reading frame of the virus associated with the acquired immune deficiency syndrome, human T-lymphotropic virus type III.
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与获得性免疫缺陷综合征相关的病毒 3 开放阅读框多态性，人类 T 淋巴细胞病毒 III 型。
DOI：
10.1093/nar/13.22.8219
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发表时间：
1985
影响因子：
14.9
通讯作者：
Arya,SK
中科院分区：
文献类型：
--
作者：
Ratner,L;Starcich,B;Josephs,SF;Hahn,BH;Reddy,EP;Livak,KJ;PettewayJr,SR;Pearson,ML;Haseltine,WA;Arya,SK
与获得性免疫缺陷综合征（AIDS）相关的病毒，人类嗜T淋巴细胞病毒III型（HTLV-III）的基因组包括两个开放阅读框架，在其他逆转录病毒中未发现。其中一个3′开放阅读框（3′ orf）长648 bp，与3′长末端重复序列（LTR）重叠。测定了其他HTLV-III克隆的序列，以估计3′orf内变异的水平和位置，从而对其蛋白产物的功能有一些了解。报告了两个未整合的HTLV-III克隆和三个由病毒体RNA制成的cDNA克隆的3′orf的新测定序列，这些病毒体RNA来自感染不同AIDS或AIDS相关复杂症状（ARC）患者的混合血样的同一细胞系。此外，3′orf的序列来源于未整合的病毒克隆，该病毒克隆来源于用来自单个患者的不同分离株感染的不同细胞系。这些序列进行了比较，以前报道的其他六个病毒克隆。序列或3′ORF在克隆间的差异为1.1- 10.4%bp和2.4-17.0%的预测氨基酸。这代表了比在整个基因组中平均发现的序列变异显著更大的序列变异。此外，功能性前病毒克隆在该开放阅读框的氨基酸残基124处具有终止密码子。这引起了关于3′ORF编码的蛋白质的结构和表达调控的问题。
The genome of the virus associated with the acquired immune deficiency syndrome (AIDS), human T-lynphotropic virus type III (HTLV-III), includes two open reading frames, not found in other retroviruses. One of these, designated 3′ open reading frame (3′ orf) is 648 base pairs (bp) in length, and overlaps with the 3′long terminal repeat (LTR) sequences. Sequences of additional HTLV-III clones were determined in order to estimate the level and location of variation within 3′orf, to gain some insight into the function of its protein product. Newly determined sequences are reported for 3′orf of two unintegrated clones of HTLV-III and three cDNA clones made from virion RNA derived from the same cell line infected with pooled blood samples of different patients with AIDS or AIDS-related complex symptoms (ARC). In addition, sequences for 3′orf were derived from an unintegrated viral clone derived from a different cell line infected with a distinct isolate from a single patient. These sequences are compared to those previously reported for six other viral clones. Sequences or 3′orf differ among clones by 1.1–10.4% bp and 2.4–17.0% of predicted amino acids. This represents significantly greater sequence variation than is found in the entire genome on average. Moreover, a functional proviral clone has a termination codon at amino acid residue 124 of this open reading frame. This raises questions concerning the structure, and regulation of expression of the protein encoded by 3′orf.