Osteogenic cell sheets reinforced with photofunctionalized micro-thin titanium
Osteogenic cell sheets reinforced with photofunctionalized micro-thin titanium
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DOI：
10.1177/0885328214567693
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发表时间：
2015-05-01
影响因子：
2.9
通讯作者：
Ogawa, Takahiro
中科院分区：
文献类型：
--
作者：
Ishijima, Manabu;Hirota, Makoto;Ogawa, Takahiro
细胞片层技术已用于递送具有完整细胞外基质的单层形式的细胞，用于软组织修复和再生。在这里，我们假设钛增强的细胞片可以构建骨组织工程和再生。制备了50 μ m厚的含孔钛板，并通过酸蚀进行粗糙化，其中一些经12min的UV光处理进行光功能化。通过在温度响应培养皿上培养大鼠颅骨骨膜来源的细胞并附着于钛板来制备细胞片。钛增强成骨细胞片结构取决于各种技术和材料因素：细胞片需要双面并夹在钛板之间，钛板需要超薄并含有孔，以允许两个细胞片紧密贴壁。至关重要的是，钛板需要进行紫外光功能化，以确保细胞片的粘附和保留。在培养的4天内，制成的钛上的单面细胞片或双面细胞片收缩并变形。光功能化钛上的钛增强细胞片结构稳定至少长达14天，形成了预期的成骨表型（ALP产生和矿化），并保持了结构完整性而没有功能降解。钛增强成骨细胞片的成功构建与增加的细胞附着、保留和黏着斑蛋白（一种通过光功能化的粘附蛋白）的表达相关。本研究确定了构建钛增强成骨细胞片的技术和材料要求。未来的体内研究是必要的，以测试这些钛加强细胞片作为稳定的可移植性，机械耐用，形状可控的成骨装置。
Cell sheet technology has been used to deliver cells in single-sheet form with an intact extracellular matrix for soft tissue repair and regeneration. Here, we hypothesized that titanium-reinforced cell sheets could be constructed for bone tissue engineering and regeneration. Fifty-mu m-thick titanium plates containing apertures were prepared and roughened by acid etching, some of which were photofunctionalized with 12min of UV light treatment. Cell sheets were prepared by culturing rat calvarial periosteum-derived cells on temperature-responsive culture dishes and attached to titanium plates. Titanium-reinforced osteogenic cell sheet construction was conditional on various technical and material factors: cell sheets needed to be double-sided and sandwich the titanium plate, and the titanium plates needed to be micro thin and contain apertures to allow close apposition of the two cell sheets. Critically, titanium plates needed to be UV-photofunctionalized to ensure adherence and retention of cell sheets. Single-sided cell sheets or double-sided cell sheets on as-made titanium contracted and deformed within 4 days of incubation. Titanium-reinforced cell sheets on photofunctionalized titanium were structurally stable at least up to 14 days, developed the expected osteogenic phenotypes (ALP production and mineralization), and maintained structural integrity without functional degradation. Successful construction of titanium-reinforced osteogenic cell sheets was associated with increased cell attachment, retention, and expression of vinculin, an adhesion protein by photofunctionalization. This study identified the technical and material requirements for constructing titanium-reinforced osteogenic cell sheets. Future invivo studies are warranted to test these titanium-reinforced cell sheets as stably transplantable, mechanically durable, and shape controllable osteogenic devices.