A neoteric dual-signal colorimetric fluorescent probe for detecting endogenous/exogenous hydrogen peroxide in cells and monitoring drug-induced hepatotoxicity
A neoteric dual-signal colorimetric fluorescent probe for detecting endogenous/exogenous hydrogen peroxide in cells and monitoring drug-induced hepatotoxicity
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DOI：
10.1016/j.talanta.2021.122578
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发表时间：
2021-06-06
期刊：
影响因子：
6.1
通讯作者：
Sun, Ying
中科院分区：
文献类型：
--
作者：
Xu, Lanlan;Zhang, Yu;Sun, Ying
过氧化氢（H2O2）是最重要的活性氧（ROS）之一，可以在肝脏中内源性产生，并被认为是评估药物性肝损伤（DILI）的生物标志物。因此，构建一种有效的检测肝脏中H2O2的方法以评估DILI至关重要。在此，设计并合成了一种用于检测过氧化氢的新型双信号比色荧光探针XH-2。硼酸盐作为特异性识别基团被接枝到荧光团 XH-1 (Phi(F) = 0.34) 上，以建立结构上前所未有的探针。实验结果表明，探针XH-2（Phi（F）= 0.15）能够利用荧光法检测过氧化氢，具有0-140 μM（R-2 = 0.9974）的良好线性范围和91 nM（lambda（ex/em）= 570 nm/638 nm）的极低检测限。此外，该探针能够以比色方式监测过氧化氢，线性范围为0-110μM（R-2 = 0.9965）。此外，探针XH-2的特异性、血清中的适用性（98.6-109.1%）以及葡萄糖的间接检测使探针成为一种优越的探针。基于其低细胞毒性，该探针成功应用于监测内源性/外源性过氧化氢，并定量测定HepG2细胞中0-120μM（R-2 = 0.9859）范围内的过氧化氢浓度水平。最终，该探针可以有效监测 HepG2 细胞 DILI 期间过氧化氢的水平。
Hydrogen peroxide (H2O2), one of the most important reactive oxygen species (ROS), can be generated endogenously in the liver and has been deemed as a biomarker for evaluating drug-induced liver injury (DILI). Therefore, it is highly crucial to construct an effective method for detecting H2O2 in the liver in order to evaluate DILI. Herein, a neoteric dual-signal colorimetric fluorescent probe XH-2 for sensing hydrogen peroxide was engineered and synthesized. Borate was grafted as a specific recognition group onto the fluorophore XH-1 (Phi(F) = 0.34) to establish a structurally unprecedented probe. The experimental results manifested that probe XH-2 (Phi(F) = 0.15) was able to detect hydrogen peroxide using a fluorescence method with an excellent linear range of 0-140 mu M (R-2 = 0.9974) and an especially low detection limit of 91 nM (lambda(ex/em) = 570 nm/638 nm). In addition, the probe was capable of monitoring hydrogen peroxide in a colorimetric manner with the linear range of 0-110 mu M (R-2 = 0.9965). Furthermore, the specificity, applicability in serum (98.6-109.1%) and indirect detection of glucose make the probe XH-2 a superior probe. Based on its low cytotoxicity, the probe was successfully applied to monitor endogenous/exogenous hydrogen peroxide and quantitatively determine the concentration level of hydrogen peroxide at a range of 0-120 mu M (R-2 = 0.9859) in HepG2 cells. Ultimately, the probe could effectively monitor the level of hydrogen peroxide during DILI in HepG2 cells.