Inhibitory zinc-enriched terminals in mouse spinal cord
Inhibitory zinc-enriched terminals in mouse spinal cord
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DOI：
10.1016/s0306-4522(01)00243-3
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发表时间：
2001-01-01
期刊：
影响因子：
3.3
通讯作者：
Schroder, HD
中科院分区：
文献类型：
--
作者：
Danscher, G;Jo, SM;Schroder, HD
应用锌转运蛋白3和谷氨酸脱羧酶免疫组织化学和锌硒自显影技术，分别研究了锌转运蛋白3、谷氨酸脱羧酶和锌离子在小鼠脊髓富锌末梢的超微结构定位，并观察了锌硒自显影、银颗粒、和锌转运蛋白3和谷氨酸脱羧酶免疫组化斑点在腹角和背角，如在光学显微镜下看到的，对应于它们的存在于突触囊泡中，在超微结构水平上的富锌末端。富锌终端的密集人口被认为是在背角板层I，III和IV，而更深的板层V和VI包含较少的终端。在超微结构水平上，富锌终末主要在胞体和树突上形成对称性突触。在富锌末梢上只观察到相对较少的不对称突触。一般情况下，最大的富锌终末接触神经元胞体和大树突，而中型和小型终末接触小型树突。前角主要由大中型富锌终末构成，而背角则主要由中小型富锌终末构成，具有含锌离子的扁平突触囊泡和对称突触接触的终扣的存在表明哺乳动物脊髓中存在抑制性富锌终末，并且这通过锌离子和谷氨酸脱羧酶共定位在这些末端中的发现得到证实。在背角和腹角富含锌的钮的模式是兼容的证据表明，锌可能参与感觉传递和运动控制。(C)2001年IBRO。由爱思唯尔科技有限公司出版。保留所有权利。
The ultrastructural localization of zinc transporter-3, glutamate decarboxylase and zinc ions in zinc-enriched terminals in the mouse spinal cord was studied by zinc transporter-3 and glutamate decarboxylase immunohistochemistry and zinc selenium autometallography, respectively.The distribution of zinc selenium autometallographic, silver grains, and zinc transporter-3 and glutamate decarboxylase immunohistochemical puncta in both ventral and dorsal horns as seen in the light microscope corresponded to their presence in the synaptic vesicles of zinc-enriched terminals at ultrastructural levels. The densest populations of zinc-enriched terminals were seen in dorsal horn laminae I, III and IV, whereas the deeper laminae V and VI contained fewer terminals. At ultrastructural levels, zinc-enriched terminals primarily formed symmetrical synapses on perikarya and dendrites. Only relatively few asymmetrical synapses were observed on zinc-enriched terminals. In general, the biggest zinc-enriched terminals contacted neuronal somata and large dendritic elements, while medium-sized and small terminals made contacts on small dendrites. The ventral horn was primarily populated by big and medium-sized zinc-enriched terminals, whereas the dorsal horn was dominated by medium-sized and small zinc-enriched terminals.The presence of boutons with flat synaptic vesicles with zinc ions and symmetric synaptic contacts suggests the presence of inhibitory zinc-enriched terminals in the mammalian spinal cord, and this was confirmed by the finding that zinc ions and glutamate decarboxylase are co-localized in these terminals. The pattern of zinc-enriched boutons in both dorsal and ventral horns is compatible with evidence suggesting that zinc may be involved in both sensory transmission and motor control. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.