A leucine-rich repeat receptor-like protein kinase (LRPKm1) gene is induced in Malus x domestica by Venturia inaequalis infection and salicylic acid treatment
A leucine-rich repeat receptor-like protein kinase (LRPKm1) gene is induced in Malus x domestica by Venturia inaequalis infection and salicylic acid treatment
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DOI：
10.1023/a:1006275924882
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发表时间：
1999-08-01
影响因子：
5.1
通讯作者：
De Lorenzo, G
中科院分区：
文献类型：
--
作者：
Komjanc, M;Festi, S;De Lorenzo, G
一个cDNA克隆，编码一个富含亮氨酸重复序列（LRR）的受体样蛋白激酶（LRPKm 1）的苹果。用克隆的菜豆多聚半乳糖醛酸酶抑制蛋白（PGIP）基因作为异源探针，从菜豆中分离到Florina。还分离出了含有LRPKm 1基因5 '调控区和开放阅读框5'部分的基因组克隆。cDNA克隆中存在2997 nt（999个氨基酸）的开放阅读框，编码受体样蛋白，其包含用于分泌的21个氨基酸的信号肽、亮氨酸拉链、23个LRR、推定的跨膜区和丝氨酸/苏氨酸蛋白激酶结构域。LRPKm 1与A.拟南芥受体样蛋白激酶RLK 5，并在较小程度上，PGIP。LRPKm 1区域+5至+600显示了另一个阅读框架，其编码的产物对应于与几种富含羟脯氨酸的蛋白同源的富含脯氨酸的蛋白片段。Southern杂交分析表明，LRPKm 1属于一个多基因家族，且在不同M.栽培品种。北方印迹分析进行的mRNA提取的感染叶片的任何一个品种。Florina（抗黑星病菌）或CV。金冠（易受V. inaequalis），并从水杨酸处理的组织。一个3500 bp的转录本在高严格杂交与LRPKm 1的cDNA积累响应感染或水杨酸处理。在不亲和互作中，转录本积累比亲和互作更强烈。这种受体样蛋白激酶的可能参与苹果抗植物病原真菌进行了讨论。
A cDNA clone encoding a leucine-rich repeat (LRR) receptor-like protein kinase (LRPKm1) of Malus x domestica cv. Florina has been isolated using as a heterologous probe a cloned gene encoding a polygalacturonase-inhibiting protein (PGIP) of Phaseolus vulgaris L. A genomic clone containing the 5'-regulatory region and a 5' portion of the open reading frame of the LRPKm1 gene has also been isolated. An open reading frame of 2997 nt (999 amino acids) was present in the cDNA clone, encoding a receptor-like protein comprising a 21 amino acid signal peptide for secretion, a leucine zipper, 23 LRRs, a putative membrane-spanning region and a serine/threonine protein kinase domain. LRPKm1 shows homology to the A. thaliana receptor-like protein kinase RLK5 and, to a minor extent, to PGIP. The LRPKm1 region from +5 to +600 exhibits an alternative reading frame that encodes a product corresponding to a proline-rich protein fragment homologous to several hydroxyproline-rich proteins. Southern blot analysis showed that LRPKm1 belongs to a multigene family and that there is length polymorphism of the hybridizing restriction fragments among different M. x domestica cultivars. Northern blot analysis was carried out on mRNA extracted from infected leaves of either cv. Florina (resistant to Venturia inaequalis) or cv. Golden Delicious (susceptible to V. inaequalis), and from tissues treated with salicylic acid. A 3500 bp transcript hybridizing at high stringency with the LRPKm1 cDNA accumulated in response to infection or salicylic acid treatment. Transcript accumulation was more intense in the incompatible interaction than in the compatible one. The possible involvement of this receptor-like protein kinase in resistance of apple to phytopathogenic fungi is discussed.