Arginine-glycine-aspartic acid motif is critical for human parechovirus 1 entry
Arginine-glycine-aspartic acid motif is critical for human parechovirus 1 entry
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DOI：
10.1128/jvi.75.20.10000-10004.2001
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发表时间：
2001-10-01
影响因子：
5.4
通讯作者：
Stanway, G
中科院分区：
文献类型：
--
作者：
Boonyakiat, Y;Hughes, PJ;Stanway, G
用突变法研究了人双埃柯病毒1型（Parechovirus 1，HPV1）VP1的RGD基序。RGD到RGE的变化仅产生具有恢复的RGD的回复突变病毒，而GD的缺失是致命的且不可回复的。+1和+2位置的突变对生长特性有一定影响，+1 M到P的变化是致命的。这些研究表明，RGD基序在感染性中起着关键作用，可能是通过与整合素相互作用，并且下游氨基酸可以对功能产生影响。
The human parechovirus 1 RGD motif in VP1 was studied by mutagenesis. An RGD-to-RGE change gave only revertant viruses with a restored RGD, while deletion of GD was lethal and nonrevertable. Mutations at the +1 and +2 positions had some effect on growth properties and a +1 M-to-P change was lethal. These studies indicate that the RGD motif plays a critical role in infectivity, presumably by interacting with integrins, and that downstream amino acids can have an influence on function.