Differential effect of an Ig mu transgene on development of pre-B cells in fetal and adult SCID mice.
Differential effect of an Ig mu transgene on development of pre-B cells in fetal and adult SCID mice.
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Ig mu 转基因对胎儿和成年 SCID 小鼠前 B 细胞发育的不同影响。
DOI：
10.1073/pnas.96.21.11952
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发表时间：
1999
影响因子：
11.1
通讯作者：
Bosma,MJ
中科院分区：
文献类型：
--
作者：
Bosma,GC;Chang,Y;Karasuyama,H;Bosma,MJ
前B淋巴细胞向前B期的进展取决于前B细胞受体（前BCR）的表达，前BCR由IG μ H链、IG替代轻链和相关信号转导链组成。不能表达前BCR的小鼠显示B细胞发育在前B阶段的停滞。这是严重联合免疫缺陷（SCID）小鼠的情况，其中由于V（D）J重组的缺陷而没有产生μ链。当产生μ链时，如在携带功能性μ转基因的SCID小鼠中，则B细胞分化可以进行到前B阶段。然而，正如本文所报道的，促进成人骨髓中SCID前B细胞发育的μ转基因（M54）在胎儿肝脏中未能做到这一点。我们认为，含有M54 μ链的前BCR不能在胎肝微环境中发出前B细胞进展到前B阶段的信号。
Progression of pro-B lymphocytes to the pre-B stage depends on the expression of a pre-B cell receptor (pre-BCR), consisting of an Ig μ H chain, Ig surrogate light chain, and associated signal transducing chains. Mice that are unable to express a pre-BCR show an arrest of B cell development at the pro-B stage. Such is the case for severe combined immune deficient (SCID) mice in which μ chains are not made because of a defect in V(D)J recombination. When μ chains are made, as in SCID mice bearing a functional μ transgene, then B cell differentiation can proceed to the pre-B stage. However, as reported here, a μ transgene (M54) that promotes development of SCID pre-B cells in adult bone marrow fails to do so in fetal liver. We suggest that a pre-BCR containing the M54 μ chain cannot signal progression of pro-B cells to the pre-B stage in the fetal liver microenvironment.