Time-course of behavioural changes induced by ethanol in zebrafish (Danio rerio)
Time-course of behavioural changes induced by ethanol in zebrafish (Danio rerio)
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DOI：
10.1016/j.bbr.2013.05.065
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发表时间：
2013-09-01
影响因子：
2.7
通讯作者：
Gerlai, Robert
中科院分区：
文献类型：
--
作者：
Steven Tran;Gerlai, Robert
斑马鱼被提议用于研究乙醇对脊椎动物大脑的影响。行为测试已成功地用于这些影响的表型表征。然而，乙醇引起的斑马鱼行为变化的短尺度（分钟到分钟）时间过程。没有被分析。目前的研究使用2 x 3慢性x急性乙醇暴露实验设计来证明这种需要。我们首先将斑马鱼暴露于乙醇长期使用剂量递增的程序，其中鱼保持在最终浓度为0.5%体积/体积乙醇10天，而对照鱼接受相同的给药程序，但没有乙醇。随后，我们将斑马鱼暴露于急性剂量的乙醇（0.00，0.50或1.00%vol/vol）中1小时，并在此期间监测其行为。我们量化的平均值和个体内的时间方差的距离旅行，距离底部和角速度使用视频跟踪，并建立时间轨迹的乙醇引起的行为变化在斑马鱼。例如，我们发现最高急性剂量组的鱼以前没有暴露于慢性乙醇，表现出一个倒U形的时间轨迹的距离旅行（双相酒精效应）。我们发现这种反应在慢性乙醇暴露后减弱（耐受性的发展）。我们还描述了一个急性乙醇戒断诱导角速度的增加。我们得出结论，斑马鱼行为的时间分析是研究慢性和急性乙醇暴露诱导的脊椎动物大脑功能变化的敏感方法（C）2013 Elsevier B. V.保留所有权利。
The zebrafish has been proposed for the study of the effects of ethanol on the vertebrate brain. Behavioural tests have been successfully employed in the phenotypical characterization of these effects. However, the short scale (minute to minute) time course of ethanol induced changes of zebrafish behaviour has. not been analyzed. The current study alleviates this need using a 2 x 3 chronic x acute ethanol exposure experimental design. We first expose zebrafish to ethanol chronically using a dose escalation procedure in which fish are kept in a final concentration of 0.5% vol/vol ethanol for 10 days while control fish receive identical dosing procedures but no ethanol. Subsequently, we expose zebrafish for 1 h to an acute dose of ethanol (0.00, 0.50, or 1.00% vol/vol) and monitor their behaviour throughout this period. We quantify the mean and within-individual temporal variance of distance travelled, distance from bottom and angular velocity using video-tracking, and establish temporal trajectories of ethanol induced behavioural changes in zebrafish. For example, we find fish of the highest acute dose group previously not exposed to chronic ethanol to exhibit an inverted U shaped temporal trajectory in distance travelled (biphasic alcohol effect). We find this response to be blunted after chronic ethanol exposure (development of tolerance). We also describe an acute ethanol withdrawal induced increase in angular velocity. We conclude that temporal analysis of zebrafish behaviour is a sensitive method for the study of chronic and acute ethanol exposure induced functional changes in the vertebrate brain (C) 2013 Elsevier B.V. All rights reserved.