Prevention of collagen-induced arthritis in mice by a polyphenolic fraction from green tea
Prevention of collagen-induced arthritis in mice by a polyphenolic fraction from green tea
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DOI：
10.1073/pnas.96.8.4524
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发表时间：
1999-04-13
影响因子：
11.1
通讯作者：
Mukhtar, H
中科院分区：
文献类型：
--
作者：
Haqqi, TM;Anthony, DD;Mukhtar, H
识别能够提供保护或调节关节炎发作和严重程度的常见膳食物质可能对人类健康具有重要影响。从绿色茶中分离出的富含抗氧化剂的多酚组分（绿色茶多酚，GTP）已被证明在实验动物中具有抗炎和抗癌特性。在三个独立的实验中，与未给予GTP水溶液的小鼠（84%至100%）相比，给予GTP水溶液的小鼠关节炎发病率显著降低（33%至50%）。关节炎指数也显着降低GTP喂养的动物。蛋白质印迹分析显示，在GTP喂养小鼠的关节炎关节中，炎症介质如环氧合酶2、IFN-γ和肿瘤坏死因子α的表达显著减少。GTP喂养的小鼠中关节炎关节的组织学和免疫组织化学分析表明，与非GTP喂养的小鼠的关节炎关节中的大量细胞浸润和完全发育的血管翳相反，IFN-γ和肿瘤坏死因子α产生细胞仅浸润边缘关节，中性内肽酶活性在非GTP喂养的小鼠的关节炎关节中比在非GTP喂养的小鼠的关节炎关节中高约7倍。在未免疫小鼠的关节炎关节中，它仅高2倍，而在GTP喂养小鼠的关节炎关节中，它仅高2倍。此外，总IgG和II型胶原蛋白特异性IgG水平较低的GTP喂养小鼠的血清和关节炎关节。综上所述，我们的研究表明，从绿色茶中提取的多酚类成分富含抗氧化剂，可能有助于预防关节炎的发作和严重程度。
Identification of common dietary substances capable of affording protection or modulating the onset and severity of arthritis may have important human health implications. An antioxidant-rich polyphenolic fraction isolated from green tea (green tea polyphenols, GTPs) has been shown to possess anti-inflammatory and anticarcinogenic properties in experimental animals, In this study we determined the effect of oral consumption of GTP on collagen-induced arthritis in mite. In three independent experiments mice given GTP in water exhibited significantly reduced incidence of arthritis (33% to 50%) as compared with mice not given GTP in water (84% to 100%). The arthritis index also was significantly lower in GTP-fed animals. Western blot analysis showed a marked reduction in the expression of inflammatory mediators such as cyclooxygenase 2, IFN-gamma, and tumor necrosis factor alpha in arthritic joints of GTP-fed mice. Histologic and immunohistochemical analysis of the arthritic joints in GTP-fed mice demonstrated only marginal joint infiltration by IFN-gamma and tumor necrosis factor alpha-producing cells as opposed to massive cellular infiltration and fully developed pannus in arthritic joints of non-GTP-fed mice, The neutral endopeptidase activity was approximately 7-fold higher in arthritic joints of non-GTP-fed mice in comparison to non-arthritic joints of unimmunized mice whereas it was only 2-fold higher in the arthritic joints of GTP-fed mice. Additionally, total IgG and type II collagen-specific IgG levels were lower in serum and arthritic joints of GTP-fed mice. Taken together our studies suggest that a polyphenolic fraction from green tea that is rich in antioxidants may be useful in the prevention of onset and severity of arthritis.