Characterization of Fluoroglycofen Ethyl Degradation by Strain Mycobacterium phocaicum MBWY-1
Characterization of Fluoroglycofen Ethyl Degradation by Strain Mycobacterium phocaicum MBWY-1
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DOI：
10.1007/s00284-011-9918-0
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发表时间：
2011-06-01
影响因子：
2.6
通讯作者：
Wang, Qingling
中科院分区：
文献类型：
--
作者：
Chen, Liwei;Cai, Tianming;Wang, Qingling
从除草剂厂土壤中分离到一株氟糖cofen乙基降解菌MBWY-1。通过对该菌株16S rRNA基因序列和形态、生理生化特征的分析，鉴定该菌株为phocaicum分枝杆菌。菌株能够以氟糖乙酯为唯一碳源生长，并在72 h内将100 mg l（-1）的氟糖乙酯降解至不可检测的水平。菌株MBWY-1降解氟糖乙酯的最佳温度和pH分别为30℃和7.0℃。在氟糖cofen乙基降解过程中产生的5种代谢物，通过质谱鉴定为{5-[2-氯-4-（三氟甲基）苯氧基]-2-硝基苯氧基}羟基乙酸、acifluorfen、5-[2-氯-4-（三氟甲基）苯氧基]-2-硝基苯甲酸盐、5-[2-氯-4-（三氟甲基）苯氧基]-2-羟基和3-氯-4-羟基苯氧基。通过代谢产物的鉴定，提出了菌株MBWY-1降解氟糖乙酯的途径。将菌株MBWY-1接种到经氟糖乙酯处理的土壤中，无论土壤是否灭菌，其氟糖乙酯降解率均高于未接种土壤。
A fluoroglycofen ethyl-degrading bacterium, MBWY-1, was isolated from the soil of an herbicide factory. This isolated strain was identified as Mycobacterium phocaicum based on analysis of its 16S rRNA gene sequence and its morphological, physiological, and biochemical properties. The strain was able to utilize fluoroglycofen ethyl as its sole source of carbon for growth and could degrade 100 mg l(-1) of fluoroglycofen ethyl to a non-detectable level within 72 h. The optimum temperature and pH for fluoroglycofen ethyl degradation by strain MBWY-1 were 30 degrees C and 7.0, respectively. Five metabolites produced during the degradation of fluoroglycofen ethyl and were identified by mass spectrometry as {5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitrophenylacyl} hydroxyacetic acid, acifluorfen, 5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-nitrobenzoate, 5-[2-chloro-4-(trifluoromethyl) phenoxy]-2-hydroxyl, and 3-chloro-4-hydroxyl benzotrifluoride. Identification of the metabolites allowed to propose the degradation pathway of fluoroglycofen ethyl by strain MBWY-1. The inoculation of strain MBWY-1 into soil treated with fluoroglycofen ethyl resulted in a higher fluoroglycofen ethyl degradation rate than in uninoculated soil regardless of whether the soil was sterilized or nonsterilized.