Isolation and characterization of the rat prolactin-releasing peptide gene: multiple TATA boxes in the promoter region.
Isolation and characterization of the rat prolactin-releasing peptide gene: multiple TATA boxes in the promoter region.
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大鼠催乳素释放肽基因的分离和表征：启动子区域的多个 TATA 盒。
DOI：
10.1006/bbrc.2001.4308
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发表时间：
2001
影响因子：
3.1
通讯作者：
M. Mori
中科院分区：
文献类型：
--
作者：
M. Yamada;A. Ozawa;S. Ishii;N. Shibusawa;T. Hashida;T. Ishizuka;T. Hosoya;T. Monden;T. Satoh;M. Mori
催乳素释放肽（Prolactin-releasing peptide，PrRP）基因是一种新的生物活性肽，表达于脑内非常有限的区域。为了探讨PrRP基因表达的分子机制，我们克隆了PrRP基因及其启动子区，并对其进行了结构分析。该基因跨度约2.4 kb，包含三个外显子和两个内含子。3 'RACE分析显示终止密码子下游103 bp处的多聚腺苷酸化信号是功能性的。引物延伸分析表明，三个转录起始位点（TSS）92，199，和325 bp的翻译起始位点上游。有趣的是，除了SP1-1、AP-2和Oct-2A的假定结合位点之外，还在这些TSS附近鉴定了三个特征性TATA盒。利用一系列缺失突变体的瞬时转染研究表明，中间TATA盒对启动子活性是重要的。此外，克隆的1.6kb启动子区仅在神经元和垂体来源的细胞系中有活性，并且启动子区-1600约-800bp作为负调控元件工作。我们首次证明了PrRP基因的基因组结构和启动子功能，这些知识将有助于阐明PrRP基因的转录控制。
The prolactin-releasing peptide (PrRP) gene is a novel bioactive peptide expressed in very restricted regions in the brain. To explore the molecular mechanism of PrRP gene expression, we cloned and characterized the gene and its promoter region. The gene spans approximately 2.4 kb and contains three exons and two introns. 3'RACE analysis showed that a polyadenylation signal 103 bp downstream from the stop codon was functional. Primer extension analysis indicated three transcriptional start sites (TSSs) 92, 199, and 325 bp upstream from the translational start site. Interestingly, in addition to the putative binding sites for SP1-1, AP-2, and Oct-2A, three characteristic TATA boxes were identified close to these TSSs. Transient transfection study using a series of deletion mutants revealed that the middle TATA box is important for the promoter activity. Furthermore, the cloned 1.6 kb promoter region was active only in neuron- and pituitary-derived cell lines, and the promoter region -1600 approximately -800 bp worked as a negative regulatory element. We demonstrated for the first time, the genomic organization and promoter function of the PrRP gene, and this knowledge will facilitate elucidation of transcriptional control of the PrRP gene.
编码人前促甲状腺素释放激素的人基因组 DNA 和下丘脑 cDNA 的克隆和结构。
DOI：
10.1210/mend-4-4-551
发表时间：
1990
期刊：
Molecular endocrinology (Baltimore, Md.)
影响因子：
--
作者：
Yamada,M;Radovick,S;Wondisford,FE;Nakayama,Y;Weintraub,BD;Wilber,JF
通讯作者：
Wilber,JF
催乳素释放肽及其受体信使核糖核酸在正常人垂体和垂体腺瘤中的表达。
DOI：
10.1210/jcem.84.12.6191
发表时间：
1999
期刊：
The Journal of clinical endocrinology and metabolism.
影响因子：
--
作者：
Zhang,X;Danila,DC;Katai,M;Swearingen,B;Klibanski,A
通讯作者：
Klibanski,A
影响因子：
4.4
作者：
MARCHESE, A;HEIBER, M;DOCHERTY, JM
通讯作者：
DOCHERTY, JM