Measurement of clonazepam by electron-capture gas-liquid chromatography with application to single-dose pharmacokinetics.
Measurement of clonazepam by electron-capture gas-liquid chromatography with application to single-dose pharmacokinetics.
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电子捕获气相色谱法测定氯硝西泮并应用于单剂量药代动力学。
DOI：
10.1093/jat/11.2.55
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发表时间：
1987
影响因子：
2.5
通讯作者：
Greenblatt,DJ
中科院分区：
文献类型：
--
作者：
Miller,LG;Friedman,H;Greenblatt,DJ
建立了一种简便、灵敏、快速的血中氯硝西泮的气液色谱-电子捕获检测法。该方法采用中性苯从血浆中提取并浓缩至小体积，用于自动进样至配备电子捕获检测器的色谱仪中。该方法在氯硝西泮和去甲基氟硝西泮（内标物）保留时间对应的区域始终提供不含污染物的空白血浆样品。灵敏度为1 ng/mL，标准曲线在1 - 200 ng/mL范围内呈线性。说明了该方法对单剂量药代动力学的适用性。
A simple, sensitive, and rapid method for analysis of clonazepam in blood by gas-liquid chromatography with electron-capture detection is described. The method employs neutral benzene extraction from plasma and concentration into a small volume for autoinjection into the electron capture detector-equipped chromatograph. This method consistently provides blank plasma samples free of contaminants in the areas corresponding to the retention times for clonazepam and desmethylflunitrazepam (the internal standard). Sensitivity is 1 ng/mL, and standard curves are linear from 1 to 200 ng/mL. The applicability of the method to single-dose pharmacokinetics is illustrated.