Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA.
Specific Neisseria gonorrhoeae DNA-probes derived from ribosomal RNA.
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源自核糖体 RNA 的特定淋病奈瑟氏菌 DNA 探针。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
H. V. Heuverswijn
中科院分区：
文献类型：
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作者：
R. Rossau;E. Vanmechelen;J. D. Ley;H. V. Heuverswijn
对淋球菌16S和23S核糖体核糖核酸(RRNA)非保守区的18个序列进行了亚克隆或化学合成，并以斑点脱氧核糖核酸(DNA)：DNA杂交法作为探针。其中一些探针只检测到淋球菌的核酸，而另一些探针也显示了与其他细菌物种的核酸的杂交信号。我们的结果表明，rRNA衍生的DNA探针可以用来区分非常接近的类群，而不需要使用Southern-Blot分析。
Eighteen sequences complementary to less-conserved regions within the 16S and 23S ribosomal ribonucleic acid (rRNA) of Neisseria gonorrhoeae were subcloned or chemically synthesized and used as probes in a dot-spot deoxyribonucleic acid (DNA): DNA hybridization format. Some of these probes exclusively detected Neisseria gonorrhoeae nucleic acid, whereas others also showed hybridization signals with nucleic acid from other bacterial species. Our results indicate that rRNA-derived DNA-probes can be used to differentiate between very closely related taxa without the use of Southern-blot analysis.