Cross-reactivity among edible nuts: double immunodiffusion, crossed immunoelectrophoresis and human specitic IgE serologic surveys
Cross-reactivity among edible nuts: double immunodiffusion, crossed immunoelectrophoresis and human specitic IgE serologic surveys
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DOI：
10.1016/s1081-1206(10)61187-8
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发表时间：
2005-07-01
影响因子：
5.9
通讯作者：
Goetz, AD
中科院分区：
文献类型：
--
作者：
Goetz, DW;Whisman, BA;Goetz, AD
背景：多达三分之一的食物过敏原过敏事件与坚果摄入有关。虽然并发过敏原敏感树nuts是常见的，坚果抗原之间的交叉反应性是不太好defined.Objective：调查7树nuts（核桃，山核桃，榛子，腰果，巴西坚果，开心果，杏仁）和花生之间的血清学交叉反应性。结果：坚果特异性IgE酶联免疫吸附试验（ELISA）显示，花生与树坚果间无交叉反应。在树坚果中，定义了2组过敏原交叉反应性：（1）核桃、山核桃和榛子;（2）榛子、腰果、巴西坚果、开心果和杏仁。双重免疫扩散，交叉线免疫电泳，交叉免疫电泳与中间凝胶的结果支持相同的分组的交叉反应树坚果和确定几个不太突出的坚果坚果抗原交叉反应组之间和与peanut.Conclusion：除了少数例外（特别是有限的花生与开心果和核桃的交叉反应），花生抗原没有血清学交叉反应树坚果。核桃、山核桃和榛子形成一组强烈交叉反应的树坚果。榛子、腰果、巴西坚果、开心果和杏仁形成一组适度交叉反应的树坚果。这些组之间的交叉反应不太明显（特别是核桃和山核桃与巴西坚果的交叉反应有限）。树坚果之间最强的交叉反应遵循植物科协会：（1）胡桃科的核桃和山核桃，（2）漆树科的腰果和阿月浑子。
Background: As many as one third of all food allergen anaphylactic events are related to tree nut ingestion. Although concurrent allergen sensitivity to tree nuts is common, cross-reactivity among nut antigens is less well defined.Objective: To survey serologic cross-reactivities among 7 tree nuts (walnut, pecan, hazelnut, cashew, Brazil nut, pistachio, and almond) and peanut.Methods: Human specific IgE enzyme-linked immunosorbent assay inhibition was used to identify nut allergen cross-reactivities. Single-nut rabbit antisera were used in double immunodiffusion, crossed-line immunoelectrophoresis, and crossed immunoelectrophoresis with intermediate gel studies of nut antigen cross-reactivity.Results: Nut specific IgE enzyme-linked immunosorbent assay inhibition demonstrated no cross-reactivities between peanut and tree nuts. Among tree nuts, 2 groups with allergen cross-reactivity were defined: (1) walnut, pecan, and hazelnut and (2) hazelnut, cashew, Brazil nut, pistachio, and almond. Double immunodiffusion, crossed-line immunoelectrophoresis, and crossed immunoelectrophoresis with intermediate gel results supported the same groupings of cross-reactive tree nuts and identified several less prominent nut-nut antigen cross-reactivities between groups and with peanut.Conclusion: With few exceptions (notably limited peanut cross-reactivity with pistachio and walnut), peanut antigens did not serologically cross-react with tree nuts. Walnut, pecan, and hazelnut form a group of strongly cross-reactive tree nuts. Hazelnut, cashew, Brazil nut, pistachio, and almond form a group of moderately cross-reactive tree nuts. Cross-reactivities between these groups are less pronounced (notably limited cross-reactivity of walnut and pecan with Brazil nut). The strongest cross-reactivities among tree nuts follow botanical family associations: (1) walnut and pecan in the family Juglandaceae and (2) cashew and pistachio in the family Anacardiaceae.