Determination of ventilatory volume in mice by whole body plethysmography
Determination of ventilatory volume in mice by whole body plethysmography
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DOI：
10.2170/jjphysiol.47.317
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发表时间：
1997-08-01
期刊：
影响因子：
--
通讯作者：
Masuda, Y
中科院分区：
文献类型：
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作者：
Onodera, M;Kuwaki, T;Masuda, Y
我们检查了全身体积描记法（WBP）用于确定小鼠排泄体积的可靠性。使用麻醉的年轻雄性129/Sv小鼠，分析连续通过WBP测定的潮气量（VT）与通过直接体积描记法（DP）或呼吸速度描记法（PT）测定的潮气量（VT）的一致程度。WBP和DP之间VT的平均差异为0.000 ml，95%置信区间为-0.003至0.002，而WBP和PT之间的平均差异为0.000 ml（-0.004至0.003），WBP与DP的一致性限度或差异的平均值± 2SD分别为-0.008和0.008 ml，WBP与PT的一致性限度或差异的平均值± 2SD分别为-0.011和0.010 ml。这些值为平均VT的5.1-6.6%（WBP和DP为0.157 ml，WBP和PT为0.165 ml）。因此，VT可以由WBP确定，没有任何偏差，系统误差小于7%的其他直接方法测量的检查。通过对清醒的129/Sv和129/SVJ-ICR杂交小鼠应用WBP，进一步研究了小鼠品系、年龄和性别差异对呼吸频率（f）和每分钟通气量（（V）/dot = VT）的影响。f），以及它们对全身性缺氧和高碳酸血症的反应的反射变化。(V)年轻（10-16周龄）雄性129/Sv的过点率显著高于年轻雄性129/SVJ-ICR杂种（分别为139 +/- 11和73 +/- 8 ml/min/100 g），而反射反应模式相似。老年（32-44周龄）男性（129/Sv）的（V）过点基础水平及其反射性增高均较年轻对照组降低。然而，我们无法发现老年雄性和雌性129/Sv小鼠之间的显著差异。总之，WBP是一个有用的和可靠的方法来确定清醒和麻醉小鼠的呼吸参数，此外，有相当大的应变和年龄相关的基线通气和反射反应缺氧和高碳酸血症的差异。
We examined the reliability of whole-body plethysmography (WBP) for determining the ventilatory volume in mice. The degree of agreement of tidal volume (VT) determined consecutively by WBP with that by direct plethysmography (DP) or pneumotachography (PT) was analyzed using urethane-anesthetized young male 129/Sv mice. Between WBP and DP, the mean difference of VT was 0.000 ml with a 95% confidence interval of -0.003 to 0.002, whereas that between WBP and PT was 0.000 ml (-0.004 to 0.003), The limits of agreement, or mean +/-2SD of the difference, were -0.008 and 0.008 ml in WBP vs, DP, and -0.011 and 0.010 ml in WBP vs. PT. These values were 5.1-6.6% of the average VT (0.157 ml in WBP and DP, and 0.165 ml in WBP and PT). Thus, VT can be determined by WBP without any bias and with a systemic error of less than 7% of that measured by the other direct methods examined. Through applying WBP to conscious 129/Sv and 129/SVJ-ICR hybrid mice, we further studied the effects of differences in mouse strain, age and sex on respiratory rate (f) and minute volume ((V) over dot = VT . f), and their reflex changes in responses to systemic hypoxia and hypercapnia. (V) over dot was significantly greater in young (10-16 weeks old) male 129/Sv than in young male 129/SVJ-ICR hybrids (139 +/- 11 and 73 +/- 8 ml/min/100 g, respectively), while the pattern of reflex responses was similar. In aged (32-44 weeks old) male 129/Sv, the basal level of (V) over dot and its reflex increases were all diminished as compared to those in their young counter parts. However, we were unable to find significant differences between aged male and female 129/Sv mice. In conclusion, WBP is a useful and reliable method to determine ventilatory parameters in conscious and anesthetized mice, Furthermore, there are considerable strain-and age-related differences in baseline ventilation and reflex responses to hypoxia and hypercapnia.