Pharaoh Cuttlefish, Sepia pharaonis, Genome Reveals Unique Reflectin Camouflage Gene Set
Pharaoh Cuttlefish, Sepia pharaonis, Genome Reveals Unique Reflectin Camouflage Gene Set
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DOI：
10.3389/fmars.2021.639670
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发表时间：
2021-02
期刊：
影响因子：
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通讯作者：
Weiwei Song;Ronghua Li;Yun Zhao;H. Migaud;Chunlin Wang;Michaël Bekaert
中科院分区：
文献类型：
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作者：
Weiwei Song;Ronghua Li;Yun Zhao;H. Migaud;Chunlin Wang;Michaël Bekaert
法老乌贼（Sepia pharaonis）是分布于我国东南沿海的一种极具经济价值的乌贼，也是世界渔业的重要资源。由于缺乏高质量的参考基因组，开发连锁图谱或标记辅助选择的研究工作受到阻碍。为了满足这一需要，我们制备了S. pharaonis使用长读段平台（Oxford Nanopore Technologies PromethION）组装基因组和短读段高质量技术（Illumina HiSeq X Ten）校正测序错误。将基因组组装成5，642个支架，总长度为4.79 Gb，支架N50为1.93 Mb。S. pharaonis基因组组装鉴定了总共51，541个基因，其中包括反射蛋白基因的12个拷贝，这些基因使乌贼能够控制它们的身体颜色。这个新的参考基因组为S。pharaonis为今后研究该物种的生物学、驯化和选择性繁殖提供了重要资源。
Sepia pharaonis, the pharaoh cuttlefish, is a commercially valuable cuttlefish species across the southeast coast of China and an important marine resource for the world fisheries. Research efforts to develop linkage mapping, or marker-assisted selection have been hampered by the absence of a high-quality reference genome. To address this need, we produced a hybrid reference genome of S. pharaonis using a long-read platform (Oxford Nanopore Technologies PromethION) to assemble the genome and short-read, high quality technology (Illumina HiSeq X Ten) to correct for sequencing errors. The genome was assembled into 5,642 scaffolds with a total length of 4.79 Gb and a scaffold N50 of 1.93 Mb. Annotation of the S. pharaonis genome assembly identified a total of 51,541 genes, including 12 copies of the reflectin gene, that enable cuttlefish to control their body coloration. This new reference genome for S. pharaonis provides an essential resource for future studies into the biology, domestication and selective breeding of the species.