STUDIES ON THE MECHANISM OF CAPACITATION - CHANGES IN PLASMA-MEMBRANE PROTEINS OF RAT SPERMATOZOA DURING INCUBATION INVITRO
STUDIES ON THE MECHANISM OF CAPACITATION - CHANGES IN PLASMA-MEMBRANE PROTEINS OF RAT SPERMATOZOA DURING INCUBATION INVITRO
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DOI：
10.1016/0006-291x(79)92092-8
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发表时间：
1979-01-01
影响因子：
3.1
通讯作者：
GERGELY, AF
中科院分区：
文献类型：
--
作者：
DAVIS, BK;GERGELY, AF
大鼠尾部-附睾精子的质膜在克雷布斯林格碳酸氢盐培养基中孵育期间发生广泛的多肽水解。加入4 mg牛血清白蛋白[BSA]/ml培养基可显著增加水解。胰蛋白酶样酶活性的表达，归因于顶体内容物的释放，在含有BSA的培养基中的精子细胞中更高。
Plasma membrane from cauda-epididymal rat spermatozoa underwent extensive polypeptide hydrolysis during incubation in Krebs Ringer bicarbonate medium. Hydrolysis was markedly increased by addition of 4 mg bovine serum albumin[BSA]/ml of medium. Expression of trypsin-like enzyme activity, attributed to release of acrosome contents, was higher in sperm cells from BSA containing medium.